Frequency of conjugative transfer of plasmid-encoded ISEcp1 - blaCTX-M-15 and aac(6')-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon - role of transferases.

Annals of Clinical Microbiology and Antimicrobials
Mohamad HarajlyGhassan M Matar

Abstract

The frequency of transfer of genes encoding resistance to antimicrobial agents was determined by conjugation in ESBL-producing and/or fluoroquinolone or aminoglycoside resistant Enterobacteriaceae clinical isolates at a tertiary care center in Lebanon. In addition, the role of tra genes encoding transferases in mediating conjugation was assessed. Conjugation experiments were done on 53 ESBL-producing and/or fluoroquinolone resistant E. coli and K. pneumoniae and ESBL-producing S. sonnei isolates. Antimicrobial susceptibility testing on parent and transconjugant isolates, and PCR amplifications on plasmid extracts of the resistance-encoding genes: blaCTX-M-15 with the ISEcp1 insertion sequence, the aac(6')-lb-cr and qnrS genes, as well as tra encoding transferases genes were done. Random amplified polymorphic DNA (RAPD) analysis was performed to demonstrate whether conjugative isolates are clonal and whether they are linked epidemiologically to a particular source. Antimicrobial susceptibility testing on transconjugants revealed that 26 out of 53 (49%) ESBL-producing Enterobacteriaceae were able to transfer antimicrobial resistance to the recipients. Transfer of high-level resistance to the transconjugants encoded by the blaCTX-...Continue Reading

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Citations

May 7, 2014·The Journal of Antimicrobial Chemotherapy·G C A AmosE M Wellington
Jul 23, 2014·Laboratory Medicine·Shahin Najar PeerayehSafura Derakhshan
Mar 19, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Tania Nawfal DagherJean-Marc Rolain
Dec 12, 2020·Antimicrobial Resistance and Infection Control·Ebtisam S MohamedSoha S Abdelrahim

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Datasets Mentioned

BETA
FJ815288

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BETA
acetylating
PCR

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