Abstract
A nested PCR and direct sequencing methods were used to define human immunodeficiency virus type 1(HIV-1) reverse transcriptase codons 41 to 219 in DNA from 127 peripheral blood mononuclear cell samples obtained from 35 patients treated with nucleoside reverse transcriptase inhibitors (NRTI). The follow-up period after the initiation of NRTI therapy was 61.8 +/- 31 months (mean and standard deviation). In addition to NRTI therapy, 32 of 35 patients were simultaneously treated with Korean red ginseng. The annual decrease in the CD4(+) T-cell count over 5 years was 13.2/microl. Twenty-eight (80%) of the 35 patients had mutations conferring resistance to NRTI. The frequencies of K70R, T215S/Y/F (i.e., mutation of T at codon 215 to S, Y, or F), D67N/E, K219Q, T69N/S/A, M41L, and L210W mutations conferring resistance to zidovudine were 57.6, 36.4, 36.4, 27.2, 24.2, 21.2, and 12.1%, respectively. Mutations conferring resistance to didanosine and lamivudine were detected in 2 (L74V and M184I; 14.2%) of 11 patients tested and in 4 (M184V; 57%) of 7 patients tested, respectively. In particular, the frequency of T69N/S/A increased sharply after more than 48 months of zidovudine monotherapy. However, Q151M was not detected. As the first r...Continue Reading
References
Jan 1, 1992·Antimicrobial Agents and Chemotherapy·J E FitzgibbonD T Dubin
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P KellamB A Larder
Feb 1, 1991·AIDS·B A LarderS D Kemp
Mar 31, 1989·Science·B A LarderD D Richman
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T ShirasakaR Yarchoan
Mar 1, 1994·The Journal of Infectious Diseases·J WahlbergJ Albert
Jan 19, 1996·Science·B F HaynesA S Fauci
Sep 1, 1996·Journal of Virology·P R HarriganB A Larder
Jun 1, 1996·European Journal of Clinical Investigation·M LealF Luque
May 21, 1998·The Journal of Infectious Diseases·Y MaedaH Mitsuya
Jun 2, 1998·The Journal of Infectious Diseases·M F KavlickH Mitsuya
Jul 11, 1998·JAMA : the Journal of the American Medical Association·L K Schrager, M P D'Souza
Nov 6, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·P OrlandiP Rossi
May 19, 1997·The American Journal of Medicine·D L Mayers
Apr 20, 1999·AIDS·J J de JongS Jurriaans
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·K HertogsB A Larder
Feb 24, 2001·Life Sciences·T B Ng, H Wang
Jul 14, 2001·Antimicrobial Agents and Chemotherapy·M A Winters, T C Merigan
Jul 20, 2001·International Immunopharmacology·Y K ChoG J Cho
Nov 16, 2001·AIDS Research and Human Retroviruses·H SungY K Cho
Citations
Mar 4, 2011·Applied Biochemistry and Biotechnology·Srividya ShivakumarJayasmita Dutta
Aug 2, 2003·AIDS Research and Human Retroviruses·Heungsup SungYoung K Cho
Oct 27, 2007·AIDS Research and Human Retroviruses·Heungsup SungYoung K Cho
Feb 21, 2008·AIDS Research and Human Retroviruses·Young-Keol Cho, You-Sun Jung
Mar 27, 2009·AIDS Research and Human Retroviruses·Young Keol ChoHeungsup Sung
Nov 11, 2010·AIDS Research and Human Retroviruses·Young-Keol ChoBrian T Foley
Mar 20, 2013·AIDS Research and Human Retroviruses·Young-Keol ChoBrian T Foley
Oct 18, 2016·Journal of Ginseng Research·Kyungtaek ImHyeyoung Min
Feb 15, 2007·Vox Sanguinis·Y K ChoJ H Kim
Apr 25, 2007·Virology·Marleen C D G HuigenMonique Nijhuis
Apr 6, 2004·Virology·John G JuliasStephen H Hughes
Apr 18, 2017·Journal of Ginseng Research·Young-Keol Cho, Jung-Eun Kim
Oct 11, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ju-Yeon ChoiSung Soon Kim
Jun 19, 2009·Clinical and Vaccine Immunology : CVI·Heungsup SungYoung-Keol Cho
Apr 9, 2005·Clinical and Diagnostic Laboratory Immunology·Heungsup SungYoung-Keol Cho