Frequency of shedding of respiratory pathogens in horses recently imported to the United States

Journal of Veterinary Internal Medicine
Fauna Leah SmithNicola Pusterla

Abstract

Imported horses that have undergone recent long distance transport might represent a serious risk for spreading infectious respiratory pathogens into populations of horses. To investigate the frequency of shedding of respiratory pathogens in recently imported horses. All imported horses with signed owner consent (n = 167) entering a USDA quarantine for contagious equine metritis from October 2014 to June 2016 were enrolled in the study. Prospective observational study. Enrolled horses had a physical examination performed and nasal secretions collected at the time of entry and subsequently if any horse developed signs of respiratory disease during quarantine. Samples were assayed for equine influenza virus (EIV), equine herpesvirus type-1, -2, -4, and -5 (EHV-1, -2, -4, -5), equine rhinitis virus A (ERAV), and B (ERBV) and Streptococcus equi subspecies equi (S. equi) using quantitative PCR (qPCR). Equine herpesviruses were detected by qPCR in 52% of the study horses including EHV-2 (28.7%), EHV-5 (40.7%), EHV-1 (1.2%), and EHV-4 (3.0%). Clinical signs were not correlated with being qPCR-positive for EHV-4, EHV-2, or EHV-5. None of the samples were qPCR-positive for EIV, ERAV, ERBV, and S. equi. The qPCR assay failed quality cont...Continue Reading

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Citations

Jun 13, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Alais Maria Dall AgnolAmauri Alcindo Alfieri
Sep 15, 2021·Equine Veterinary Journal·Charlotte Easton-Jones

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Methods Mentioned

BETA
blood draw
PCR
nucleic acid extraction
lavage

Software Mentioned

VassarStat

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