Frequency of Toxoplasma gondii in the retina in eye banks in Brazil

Parasitology Research
Deise F CostaAlessandra G Commodaro

Abstract

Ocular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus (n = 60), Sao Paulo (n = 60), Chapeco (n = 26), and Joinville (n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies: Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).

References

Aug 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J G Montoya, J S Remington
Mar 10, 2001·American Journal of Ophthalmology·C SilveiraG N Holland
Jul 31, 2003·American Journal of Ophthalmology·Claudio SilveiraRubens Belfort
Mar 1, 2005·American Journal of Ophthalmology·Adriana Lima VallochiLuiz Vicente Rizzo
Mar 7, 2006·Journal of Clinical Microbiology·S CassaingJ F Magnaval
May 19, 2006·Emerging Infectious Diseases·Asis KhanL David Sibley
Jan 29, 2011·Arquivos brasileiros de oftalmologia·Cynthia Azeredo CordeiroAntônio Lúcio Teixeira Júnior
Mar 18, 2016·Emerging Infectious Diseases·Alessandra G CommodaroMichael E Grigg

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Citations

Aug 8, 2018·Ocular Immunology and Inflammation·Marcelo RudzinskiSilvia Reina
Jul 28, 2020·Progress in Retinal and Eye Research·Justine R SmithJoão M Furtado
Jun 13, 2021·Acta Tropica·Débora de Almeida AloiseValter Ferreira de Andrade-Neto

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Methods Mentioned

BETA
PCR

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