PMID: 6971847Apr 1, 1981Paper

Frequency of U.V. induced protein-DNA crosslinks in cell lines of different sensitivities

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
K Gregg, J Mansbridge

Abstract

We have measured U.V.-induced protein-DNA crosslinking in two cultured human cell lines of different sensitivities. Using a previously published method, involving SDS-protein precipitation, we obtained a biphasic response with an initial slope of 0.6 per cent DNA J-1 m2 up to 50 J m-2 and a second-phase slope of 0.12 per cent DNA J-1 m2 with a background of 22 +/- 13 per cent. Rigorous washing of SDS-protein precipitates reduced background binding to about 5 per cent with a linear U.V. effect up to 100 J m-2 of 0.038 per cent DNA J-1 m2. Binding was judged to be covalent on the grounds of stability to boiling and represented 4.1 crosslinks pg DNA-1 J-1 m2 or 60 crosslinks cell-1 J-1 m2. Similar results were obtained for both cell lines. We conclude that the differences in U.V. survival between cell lines is not related to the extent of protein-DNA crosslinking. We have been unable to detect repair of these lesions in either cell line.

References

Nov 1, 1977·Analytical Biochemistry·J Kapuściński, B Skoczylas
Jun 1, 1976·Photochemistry and Photobiology·A Kornhauser
Dec 13, 1976·Biochimica Et Biophysica Acta·R W Clark, C S Lange
Apr 1, 1975·Photochemistry and Photobiology·A Braun, B Merrick
Nov 9, 1972·Biochimica Et Biophysica Acta·A Markovitz
Jun 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·J MARMUR, L GROSSMAN

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Citations

Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J M GaleM J Smerdon

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