PMID: 8605118Dec 1, 1995Paper

Frequent deletions of material from chromosome arm 1p in oligodendroglial tumors revealed by double-target fluorescence in situ hybridization and microsatellite analysis

Genes, Chromosomes & Cancer
Naoya HashimotoJohji Inazawa

Abstract

We undertook a cytogenetic analysis of 29 human brain tumors using double-target fluorescence in situ hybridization (FISH) and focusing on chromosome arm 1p. One or more tumor suppressor genes in this arm have been suggested to be important in a variety of neuroectodermal tumors. The series included 9 oligodendrogliomas, 4 mixed gliomas, 10 astrocytomas, 4 glioblastomas, and 2 central neurocytomas. We hybridized pericentromeric (1q12) and subtelomeric (1p36) DNA probes to cell nuclei prepared from paraffin-embedded tissues and observed a strikingly high incidence of deletion of at least part of 1p in oligodendrogliomas (100%) and mixed gliomas (75%). The results of the FISH analyses were confirmed by demonstration of loss of heterozygosity for a microsatellite polymorphism in 10 of the 29 tumors. As well as supporting the feasibility of FISH for detecting allelic deletions in chromosomes from paraffin-embedded tumor samples, the alteration of 1p reported here will contribute to an understanding of the molecular genetic events in oligodendroglial tumor development.

References

Jul 25, 1979·Nucleic Acids Research·H J Cooke, J Hindley
May 1, 1991·British Journal of Cancer·D J Venter, D G Thomas
Sep 1, 1989·Cancer Genetics and Cytogenetics·G M Brodeur, C T Fong
Jun 1, 1989·Cancer Genetics and Cytogenetics·R B JenkinsG W Dewald
Jan 1, 1988·Cancer Genetics and Cytogenetics·S H BignerD D Bigner
Jun 1, 1994·Nature Genetics·G GyapayJ Weissenbach
Jan 1, 1993·Genes, Chromosomes & Cancer·C StockP F Ambros
Jan 1, 1995·Journal of Neuropathology and Experimental Neurology·J A KrausA von Deimling
Dec 1, 1994·Neurosurgery·P CelliA Fortuna
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·V P Collins, C D James
Oct 1, 1993·The American Journal of Surgical Pathology·I SalmonR Kiss

❮ Previous
Next ❯

Citations

Aug 1, 1996·Genes, Chromosomes & Cancer·M SchwabL C Amler
Sep 17, 1998·Genes, Chromosomes & Cancer·T J HulsebosE H Bijleveld
Mar 21, 2003·Cancer Genetics and Cytogenetics·Mamoru MurakamiKatsuyoshi Mineura
May 28, 2008·Oncogene·J S WeiJ Khan
Jul 16, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ellen GelpiJohannes A Hainfellner
Aug 2, 2006·Cytopathology : Official Journal of the British Society for Clinical Cytology·D TamiolakisC Simopoulos
Feb 14, 1998·Neuropathology and Applied Neurobiology·J L DarlingT Revesz
Aug 22, 2000·Journal of Neuropathology and Experimental Neurology·J M KrosH van Dekken

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.