Frequent false-negative immunohistochemical staining with IDH1 (R132H)-specific H09 antibody on frozen section control slides: a potential pitfall in glioma diagnosis

Histopathology
Akihiko YoshidaKoichi Ichimura

Abstract

Intraoperative consultation using frozen sections (FSs) is an integral component of clinical practice. As a quality control measure, FS diagnosis is subsequently compared with the findings on a FS control slide. These control slides can be used for immunohistochemistry, and the immunohistochemical performance in FS controls is known to be antibody-dependent. Isocitrate dehydrogenase (IDH) is mutated in >80% of lower-grade infiltrating gliomas in adults and in ~10% of glioblastomas, with IDH1 (R132H) being the most common mutation. IDH status is used as a major classifier of glioma. An IDH1 (R132H)-specific antibody (H09) has been accepted as a robust surrogate for genetic testing. In this study, we aimed to determine how previous freezing and thawing affects IDH1 immunohistochemistry. Thirty cases of IDH1 (R132H)-mutant diffuse glioma, which were originally assessed on FSs, were retrieved. The positive IDH1 (R132H) status of each case was previously determined with pyrosequencing and H09 immunohistochemistry on permanent sections. The FS control tissue of each case was immunostained with H09 antibody. Among 30 gliomas, 25 showed negative reactivity on FS control slides, whereas, in the remaining five, the staining was uninterpr...Continue Reading

References

Sep 12, 2000·Applied Immunohistochemistry & Molecular Morphology : AIMM·M E EdgertonK T Montone
Feb 21, 2009·The New England Journal of Medicine·Hai YanDarell D Bigner
Jan 2, 2010·Acta Neuropathologica·Sandra Camelo-PiraguaCatherine L Nutt
Feb 15, 2011·Brain Pathology·Craig HorbinskiMarina Nikiforova
Oct 1, 2011·Clinical Neuropathology·M PreusserUNKNOWN Euro-CNS Research Committee

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Citations

May 23, 2021·Pathology, Research and Practice·Sakun SantisukwongchoteShanop Shuangshoti

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