Frequent gains at chromosome 7q34 involving BRAF in pilocytic astrocytoma

Journal of Neuropathology and Experimental Neurology
Eli E BarCharles G Eberhart

Abstract

Relatively little is known about the molecular changes that promote the formation or growth of pilocytic astrocytomas. We investigated genomic alterations in 25 pilocytic astrocytomas, including 5 supratentorial and 20 posterior fossa tumors, using oligonucleotide array comparative genomic hybridization. Large changes were identified in 7 tumors and included gains of chromosomes 5, 6, and 7 and losses of chromosomes 16, 17, 19, and 22. The most common alteration was a 1.9-MB region of low-level gain at chromosome 7q34 identified in 17 of 20 posterior fossa tumors. In most tumors, the region of gain ended within the BRAF locus and encompassed only exons that encode the BRAF kinase domain. We confirmed copy number increase at the 7q34 locus using quantitative polymerase chain reaction with primers adjacent to the HIPK2, RAB19B, and BRAF genes. Western blot analysis revealed that 3 of 6 pilocytic astrocytomas with 7q34 gain contained high levels of phosphorylated extracellular signal-related kinase (ERK) and nitrogen-activated protein kinase/ERK kinase (MEK), while 1 tumor lacking 7q34 gain and 2 normal brain specimens did not. Immunohistochemical stains of a tissue microarray containing 43 pilocytic astrocytoma identified ERK pho...Continue Reading

References

Jun 1, 1990·Molecular and Cellular Biology·G HeideckerU R Rapp
Jun 1, 1995·Cancer Genetics and Cytogenetics·D P Agamanolis, J M Malone
Sep 1, 1995·Human Pathology·F V WhiteD E Schofield
Jul 17, 1998·Cancer Genetics and Cytogenetics·H Zattara-CannoniA M Vagner-Capodano
Mar 29, 2000·British Journal of Cancer·D SanoudouN Coleman
Oct 22, 2002·Journal of Neuropathology and Experimental Neurology·Katharina WimmerTorsten Pietsch
Jul 17, 2003·The Journal of Biological Chemistry·Huira Chong, Kun-Liang Guan
Jul 23, 2004·The Journal of Molecular Diagnostics : JMD·Rodney N WiltshireRoger E McLendon
Jun 28, 2005·Journal of Neuropathology and Experimental Neurology·Hiroko Ohgaki, Paul Kleihues
Jul 27, 2005·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Sergio Rosemberg, Dirce Fujiwara
Jul 5, 2006·Seminars in Pediatric Neurology·Kaleb H Yohay
Aug 10, 2006·Journal of Neuropathology and Experimental Neurology·Sandrine RoriveChristine Decaestecker
Nov 7, 2006·Journal of Neuropathology and Experimental Neurology·David T W JonesV Peter Collins
Dec 21, 2006·British Journal of Cancer·A V EspinosaM D Ringel
Jan 9, 2007·Current Opinion in Genetics & Development·Nathalie Dhomen, Richard Marais
Feb 16, 2007·The Journal of Investigative Dermatology·Barbara DessarsPierre Heimann
Jul 10, 2007·Acta Neuropathologica·David N LouisPaul Kleihues
Aug 23, 2007·Neuropediatrics·W G JanzarikH Omran
Aug 28, 2007·Journal of Cellular Biochemistry·S Sean Houshmandi, David H Gutmann
Aug 29, 2007·Oncogene·C MichaloglouD S Peeper
Sep 20, 2007·Journal of Cell Science·Howard DonningerGeoffrey J Clark
Sep 20, 2007·The Journal of Clinical Endocrinology and Metabolism·Douglas W BallBarry D Nelkin
Feb 28, 2008·Current Opinion in Oncology·Leslie A FecherKeith T Flaherty
Mar 8, 2008·The Journal of Investigative Dermatology·Roland HoubenJuergen C Becker
Apr 10, 2008·The Journal of Clinical Investigation·Stefan PfisterPeter Lichter

❮ Previous
Next ❯

Citations

Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·David T W JonesStefan M Pfister
Aug 18, 2010·Acta Neuropathologica·Markus J RiemenschneiderGuido Reifenberger
Nov 16, 2011·Acta Neuropathologica·Michel KalamaridesNancy Ratner
Apr 24, 2013·Acta Neuropathologica·Sonika DahiyaJeffrey R Leonard
Sep 18, 2009·Neurogenetics·Ana Luísa VitalAlberto Orfao
Jan 5, 2011·Journal of Neuro-oncology·José Javier OteroScott Vandenberg
Apr 29, 2010·Current Neurology and Neuroscience Reports·Adrian M DubucMichael D Taylor
Jan 19, 2013·Paediatric Drugs·Trent R HummelDavid Franz
Jun 30, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Sabine Mueller, Susan Chang
Nov 1, 2008·Cell Death and Differentiation·D Sombroek, T G Hofmann
May 25, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Gerald F ReisTarik Tihan
Jun 29, 2011·Nature Reviews. Clinical Oncology·Ching-Hon PuiAlberto S Pappo
Nov 4, 2010·Nature Reviews. Neurology·T David Bourne, David Schiff
May 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lori A RamkissoonKeith L Ligon
Mar 13, 2010·Neuro-oncology·Sarah Z RushMichael K Cooper
Apr 12, 2012·Neuro-oncology·Craig HorbinskiIan F Pollack
Oct 24, 2012·Neuro-oncology·Nicholas F Marko, Robert J Weil
Aug 23, 2011·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·Robert A AveryGrant T Liu
Apr 18, 2012·Genes & Development·Gavin P DunnWilliam C Hahn
Oct 21, 2009·Journal of Child Neurology·Angela J Sievert, Michael J Fisher
Sep 21, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Barbara S PaughSuzanne J Baker
Mar 3, 2010·Journal of Neurosurgery. Pediatrics·Claudia FariaLúcia Roque
Apr 1, 2009·International Journal of Molecular Sciences·Markus J Riemenschneider, Guido Reifenberger
Mar 8, 2014·Current Neurology and Neuroscience Reports·John-Paul KildayEric Bouffet
Jul 24, 2014·CNS Oncology·Fausto J Rodriguez, Eric H Raabe
Jan 29, 2014·Neuro-oncology·Brett J TheelerVinay K Puduvalli
Feb 18, 2014·Journal of Neuro-oncology·Gabriela Rampazzo CruzSilvia Regina Caminada Toledo
Jun 23, 2009·Acta Neuropathologica·Andrey KorshunovChristian Hartmann
Jul 20, 2014·Current Neurology and Neuroscience Reports·Marlo R Firme, Marco A Marra
Nov 6, 2012·Annual Review of Pathology·Fausto J RodriguezCharles G Eberhart
Jun 25, 2013·Acta neurochirurgica·Antonia WadePaul Leach
Jun 30, 2015·Expert Review of Anticancer Therapy·Agusti AlentornSantosh Kesari
Sep 1, 2009·Expert Opinion on Medical Diagnostics·Abderrahmane HamlatLaurent Riffaud
Dec 20, 2014·Journal of Translational Medicine·Francesca del BufaloAngela Mastronuzzi
Mar 6, 2013·Surgical Pathology Clinics·Craig Horbinski
Jan 1, 2012·FEBS Open Bio·Yosef LavivNitza Goldenberg-Cohen
Sep 24, 2010·Seminars in Diagnostic Pathology·Tarik Tihan, Michele M Bloomer
Oct 6, 2010·Seminars in Diagnostic Pathology·Christopher Dunham

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.