PMID: 9193222Jun 1, 1997Paper

Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinomas. Identification of 2 regions of loss--18q11.1-q12.3 and 18q21.1-q23

Archives of Otolaryngology--head & Neck Surgery
J W JonesT E Carey

Abstract

To determine the frequency and regions of loss on chromosome arm 18q in uncultured head and neck squamous cell carcinomas. Polymerase chain reaction amplification of DNA extracted from 18 tumor specimens (1 patient had 2 tumors) and blood samples from 17 patients with head and neck squamous cell carcinoma was performed using primers flanking 16 microsatellite repeat polymorphisms spanning most of chromosome 18q. DNA was extracted only from specimens with greater than 70% tumor nuclei. Research university. Seventeen individuals with newly diagnosed head and neck cancer. Loss of heterozygosity (LOH). There was LOH at more than 1 locus in 52% (9/ 17) of the tumors; 3 tumors had LOH at all informative markers. Four had loss at only 1 locus, raising the total with loss to 12 (75%) of 16. Loss of 18q11.1-q12.3 in 4 tumors without distal loss defines a proximal region of loss. Loss of heterozygosity affecting 18q21.1 in 1 tumor, without proximal loss and LOH for 18q21.1, 18q22, or 18q23 in 9 (52%) of 17 tumors defines a distal region of loss. Loss of heterozygosity on chromosome arm 18q is not an artifact of in vitro culture. The finding of 18q LOH in 50% to 70% tumors makes 18q an important region for study. Regions 18q11.1-q12.3 and...Continue Reading

Citations

Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Deepak KamnasaranAbhijit Guha
Mar 26, 2004·Cancer Genetics and Cytogenetics·Anat OhaliRina Zaizov
Jul 2, 2009·International Journal of Cancer. Journal International Du Cancer·Pieter J A de KoningJ Alain Kummer
Sep 18, 2009·Head & Neck·J Chad BrennerThomas E Carey
Oct 21, 1999·Biochemical and Biophysical Research Communications·P SpringG Clayman
Mar 22, 2001·Experimental Dermatology·P B Cserhalmi-FriedmanA M Christiano
Dec 3, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·S S PrimeI C Paterson
Aug 3, 2004·Genes, Chromosomes & Cancer·Satoru TakebayashiThomas E Carey
Jul 8, 2005·Genes, Chromosomes & Cancer·Josena P KunjoonjuMaria J Worsham
Dec 12, 2018·Oral Oncology·Megan L LudwigJ Chad Brenner

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