Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

PloS One
Akanksha SrivastavaRavi Kumar Asthana

Abstract

An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for anticancer potential using human colon adenocarcinoma (HT29) and human kidney adenocarcinoma (A498) cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC) eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer) and MCF-10A (normal human epithelial) exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of antican...Continue Reading

References

Jan 1, 1997·Journal of Natural Products·G M CraggK M Snader
Aug 1, 1997·Applied and Environmental Microbiology·U NübelG Muyzer
Jun 8, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C JacksonP Sambrook
Nov 17, 2001·Current Opinion in Pharmacology·A B da RochaG Schwartsmann
May 12, 2005·Journal of Agricultural and Food Chemistry·Li-chen WuIn-Wei Lu
Aug 26, 2006·The Journal of Organic Chemistry·Alberto Plaza, Carole A Bewley
Sep 29, 2006·Cellular and Molecular Life Sciences : CMLS·A CatassiP Russo
Feb 2, 2008·Astrobiology·David SchwartzmanAlex Pavlov
May 1, 2008·Journal of the American Chemical Society·Rebecca A MedinaKerry L McPhail
Dec 2, 2009·Environmental Toxicology·Catherine BernardLarelle Fabbro
Sep 3, 2010·Journal of Natural Products·Lilibeth A SalvadorHendrik Luesch
Sep 23, 2010·BMC Complementary and Alternative Medicine·Wan-Loy ChuPhaik-Eem Lim
Oct 30, 2010·Current Opinion in Biotechnology·Joshawna K NunneryWilliam H Gerwick
May 29, 2013·Drug Discovery Today·Lik Tong Tan

❮ Previous
Next ❯

Citations

Jan 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nozomi TairaShinkichi Tawata
Oct 28, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhiqiang YeGuanli Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
Assay
Fluorescence

Software Mentioned

BD FACS Software
MEGA5
FACS Diva
SPSS

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.