FRET analysis of in vivo dimerization by RNA-editing enzymes

The Journal of Biological Chemistry
Kaari A ChilibeckAndrew M MacMillan

Abstract

Members of the ADAR (adenosine deaminase that acts on RNA) enzyme family catalyze the hydrolytic deamination of adenosine to inosine within double-stranded RNAs, a poorly understood process that is critical to mammalian development. We have performed fluorescence resonance energy transfer experiments in mammalian cells transfected with fluorophore-bearing ADAR1 and ADAR2 fusion proteins to investigate the relationship between these proteins. These studies conclusively demonstrate the homodimerization of ADAR1 and ADAR2 and also show that ADAR1 and ADAR2 form heterodimers in human cells. RNase treatment of cells expressing these fusion proteins changes their localization but does not affect dimerization. Taken together these results suggest that homo- and heterodimerization are important for the activity of ADAR family members in vivo and that these associations are RNA independent.

References

Mar 1, 1995·Molecular and Cellular Biology·M A O'ConnellW Keller
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·U KimK Nishikura
Feb 1, 1996·Nature·T MelcherM Higuchi
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·A HerbP H Seeburg
Jul 15, 1997·Biochemistry·D P Morse, B L Bass
May 20, 1999·Nature·S M RueterR B Emeson
Jun 5, 2002·Annual Review of Biochemistry·Brenda L Bass
Aug 7, 2002·The Journal of Biological Chemistry·Dominic C J JaikaranAndrew M MacMillan
Mar 6, 2003·The Journal of Biological Chemistry·Dan-Sung C ChoKazuko Nishikura
Apr 1, 2003·Journal of Cell Science·Joana M P DesterroMaria Carmo-Fonseca
Jul 4, 2003·The EMBO Journal·Angela GalloMary A O'Connell
Sep 5, 2003·The Journal of Biological Chemistry·Jing-Hua YangReuven Rabinovici
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher L SansamRonald B Emeson
Nov 14, 2003·The Journal of Biological Chemistry·Qingde WangKazuko Nishikura
Dec 6, 2003·Science·Leath A Tonkin, Brenda L Bass
Jan 23, 2004·PLoS Biology·Milena Holmgren, Stefan A Schnitzer
Feb 26, 2004·Nature Structural & Molecular Biology·Michelle A Carmell, Gregory J Hannon
Jul 16, 2004·Organic & Biomolecular Chemistry·Oliver A Kent, Andrew M MacMillan
Jul 20, 2004·Nature Biotechnology·Erez Y LevanonMichael F Jantsch
Sep 3, 2004·Genome Research·Dennis D Y KimAbram Gabriel
Nov 10, 2004·PLoS Biology·Alekos AthanasiadisStefan Maas
Nov 17, 2004·Genome Research·Matthew BlowMichael R Stratton
May 20, 2005·Current Opinion in Cell Biology·Joshua DeCerbo, Gordon G Carmichael
Jun 9, 2005·The Journal of Cell Biology·Patrice VitaliAlexander Huttenhofer

❮ Previous
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Citations

Nov 25, 2011·Molecular Neurobiology·Cesare OrlandiSergio Barlati
Nov 5, 2013·Cell Reports·Isabel X WangVivian G Cheung
Mar 30, 2012·The Journal of Biological Chemistry·Juan Pablo PalaviciniJoshua J C Rosenthal
Dec 25, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cyril X GeorgeCharles E Samuel
Jul 1, 2011·Carcinogenesis·Dan DominissiniGideon Rechavi
Jun 21, 2011·Biological Reviews of the Cambridge Philosophical Society·Angela Gallo, Franco Locatelli
Mar 3, 2010·Annual Review of Biochemistry·Kazuko Nishikura
Aug 21, 2007·Evolutionary Computation·Chien-feng HuangLuis M Rocha
Nov 22, 2013·International Journal of Molecular Sciences·Sara TomaselliAngela Gallo
Oct 6, 2015·Biomolecules·Niamh MannionMary A O'Connell
Sep 19, 2015·Stress : the International Journal on the Biology of Stress·Noa Brande-EilatInna Gaisler-Salomon
Jan 16, 2016·BMC Genomics·Melanie A HuntleyRobert Gentleman
Dec 30, 2015·Nucleic Acids Research·Uma JayachandranAtlanta G Cook
Feb 11, 2015·Bioscience Reports·HuiQi HongLeilei Chen
Jul 8, 2015·Chromosoma·Mansoureh TajaddodKonstantin Licht
Jan 18, 2013·RNA Biology·Takashi ShimokawaPeter G Zaphiropoulos
Sep 4, 2016·Gastroenterology·Valerie Blanc, Nicholas O Davidson
Sep 24, 2016·Nucleic Acids Research·Elisa OrecchiniAlessandro Michienzi
Jul 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jinyao ZhangJie He
May 18, 2018·FEBS Letters·Galina Shevchenko, Kevin V Morris
Mar 5, 2010·Genetika·E V Pankratova, A G Stepchenko
Aug 24, 2017·FEBS Letters·Aparna AnantharamanKannanganattu V Prasanth
Jul 1, 2020·Nucleic Acids Research·Alexander S Thuy-BounPeter A Beal
Nov 4, 2020·Leukemia·Phaik Ju TeohWee Joo Chng
Mar 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Tony SunCharles M Rice
Apr 22, 2021·Annual Review of Virology·Christian K PfallerCharles E Samuel

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