FRET detection of Octamer-4 on a protein nanoarray made by size-dependent self-assembly.

Analytical and Bioanalytical Chemistry
Phat L TranJeong-Yeol Yoon

Abstract

An alternative approach for fabricating a protein array at nanoscale is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Fluorescent micro- and nanobeads each conjugated with different antibodies are assembled by size-dependent self-assembly (SDSA) onto nanometer wells that were created on a polymethyl methacrylate (PMMA) substrate by electron beam lithography (EBL). Antibody-conjugated beads of different diameters are added serially and electrostatically attached to corresponding wells through electrostatic attraction between the charged beads (confirmed by zeta potential analysis) and exposed p-doped silicon substrate underneath the PMMA layer. This SDSA method is enhanced by vibrated-wire-guide manipulation of droplets on the PMMA surface containing nanometer wells. Saturation rates of antibody-conjugated beads to the nanometer patterns are up to 97% under one component and 58-70% under two components nanoarrays. High-density arrays (up to 40,000 wells) could be fabricated, which can also be multi-component. Target detection utilizes fluorescence resonance energy transfer (FRET) from fluorescent beads to fluorescent-tagged secondary antibodies to Octamer-4 (Oct4), whi...Continue Reading

References

Feb 3, 1989·Science·P K HansmaC B Prater
Nov 16, 2002·Analytical Chemistry·James B Delehanty, Frances S Ligler
Nov 28, 2002·Nature Genetics·Gavin MacBeath
Mar 14, 2003·Nature·Sam Hanash
May 8, 2003·Journal of the American Chemical Society·Ki-Bum LeeChad A Mirkin
Aug 7, 2003·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Elke HausteinPetra Schwille
Mar 5, 2004·Cellular and Molecular Life Sciences : CMLS·D RingeM J Ondrechen
Jun 3, 2004·Proteomics·Michael LynchEric Henderson
Aug 23, 2005·Angewandte Chemie·Rafael A VegaChad A Mirkin
Dec 28, 2005·Nature Materials·Chun-Yang ZhangTza-Huei Wang
Feb 4, 2006·Biotechnology Progress·Tremaine Powell, Jeong-Yeol Yoon
Sep 27, 2006·Angewandte Chemie·Khalid SalaitaChad A Mirkin
Mar 23, 2007·Analytical and Bioanalytical Chemistry·Seong Ho KangEdward S Yeung
Oct 16, 2007·Drug Discovery Today·Christer Wingren, Carl A K Borrebaeck
Jul 8, 2008·Current Opinion in Biotechnology·Francisco Ciruela
Oct 17, 2008·Proteomics·Hema Prasad NarraHoward Ochman
Nov 5, 2008·Biotechnology and Bioengineering·Leonard F PeaseMichael J Tarlov
Nov 19, 2008·Journal of Biological Engineering·Jeong-Yeol Yoon, David J You
Oct 15, 2009·Materials Science & Engineering. C, Materials for Biological Applications·Tremaine B PowellJeong-Yeol Yoon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.