FRET study of membrane proteins: simulation-based fitting for analysis of membrane protein embedment and association.

Biophysical Journal
Petr V NazarovM A Hemminga

Abstract

A new formalism for the simultaneous determination of the membrane embedment and aggregation of membrane proteins is developed. This method is based on steady-state Förster (or fluorescence) resonance energy transfer (FRET) experiments on site-directed fluorescence labeled proteins in combination with global data analysis utilizing simulation-based fitting. The simulation of FRET was validated by a comparison with a known analytical solution for energy transfer in idealized membrane systems. The applicability of the simulation-based fitting approach was verified on simulated FRET data and then applied to determine the structural properties of the well-known major coat protein from bacteriophage M13 reconstituted into unilamellar DOPC/DOPG (4:1 mol/mol) vesicles. For our purpose, the cysteine mutants Y24C, G38C, and T46C of this protein were produced and specifically labeled with the fluorescence label AEDANS. The energy transfer data from the natural tryptophan at position 26, which is used as a donor, to AEDANS were analyzed assuming a helix model for the transmembrane domain of the protein. As a result of the FRET data analysis, the topology and bilayer embedment of this domain were quantitatively characterized. The resulting...Continue Reading

References

Nov 1, 1979·Biophysical Journal·P K Wolber, B S Hudson
Jan 1, 1978·Annual Review of Biochemistry·L Stryer
Dec 1, 1980·Biophysical Journal·T G Dewey, G G Hammes
Aug 29, 2001·Nature Structural Biology·G S LakshmikanthJ B Udgaonkar
Jan 12, 2002·Current Opinion in Structural Biology·A Arora, L K Tamm
Feb 5, 2002·Biochimica Et Biophysica Acta·David StoparMarcus A Hemminga
Apr 6, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·J K Amisha Kamal, Digambar V Behere
Apr 18, 2002·Current Opinion in Structural Biology·Bernadette Byrne, So Iwata
Feb 20, 2003·Protein Science : a Publication of the Protein Society·Francesca M Marassi, Stanley J Opella
Mar 14, 2003·Trends in Biochemical Sciences·Jaume TorresMontserrat Samsó
Mar 28, 2003·Biochimica Et Biophysica Acta·David StoparMarcus A Hemminga
May 29, 2003·Biophysical Journal·Claude Berney, Gaudenz Danuser
Sep 26, 2003·Biophysical Journal·Fábio FernandesMarcus A Hemminga
Mar 23, 2004·Journal of Chemical Information and Computer Sciences·Petr V NazarovMarcus A Hemminga
Apr 6, 2005·Journal of Chemical Information and Modeling·Janez StrancarMarcus A Hemminga
Sep 10, 2005·The Journal of Biological Chemistry·Werner L VosMarcus A Hemminga

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Citations

Aug 15, 2009·European Biophysics Journal : EBJ·Marcus A HemmingaDavid Stopar
Aug 12, 2009·European Biophysics Journal : EBJ·Werner L VosMarcus A Hemminga
Mar 27, 2007·Biophysical Chemistry·Valeriya M IoffeAleksey Vasilev
Aug 21, 2007·Biophysical Journal·Werner L VosMarcus A Hemminga
Jan 14, 2009·Journal of Magnetic Resonance·Janez StrancarMarcus A Hemminga
Feb 15, 2008·The EMBO Journal·Daniel AbankwaJohn F Hancock
Oct 12, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ákos I FábiánAttila Jenei
May 10, 2017·Cellular and Molecular Life Sciences : CMLS·Seyyed Abolghasem GhadamiFabrizio Chiti
Mar 30, 2017·Methods and Applications in Fluorescence·Valeriya M TrusovaNikolai Gadjev
Sep 8, 2017·Journal of Fluorescence·Galyna P GorbenkoJulian G Molotkovsky

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