Frog Skin Microbiota Vary With Host Species and Environment but Not Chytrid Infection

Frontiers in Microbiology
Ariel Kruger

Abstract

Describing the structure and function of the amphibian cutaneous microbiome has gained importance with the spread of Batrachochytrium dendrobatidis (Bd), the fungal pathogen that can cause the skin disease chytridiomycosis. Sampling amphibian skin microbiota is needed to characterize current infection status and to help predict future susceptibility to Bd based on microbial composition since some skin microbes have antifungal capabilities that may confer disease resistance. Here, I use 16S rRNA sequencing to describe the composition and structure of the cutaneous microbiota of six species of amphibians. Frog skin samples were also tested for Bd, and I found 11.8% Bd prevalence among all individuals sampled (n = 76). Frog skin microbiota varied by host species and sampling site, but did not differ among Bd-positive and Bd-negative individuals. These results suggest that bacterial composition reflects host species and the environment, but does not reflect Bd infection among the species sampled here. Of the bacterial OTUs identified using an indicator species analysis as strongly associated with amphibians, significantly more indicator OTUs were putative anti-Bd taxa than would be expected based on the proportion of anti-Bd OTUs a...Continue Reading

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Citations

Dec 16, 2020·Annual Review of Animal Biosciences·Raquel S PeixotoKaren E Nelson
May 9, 2021·Current Opinion in Microbiology·Louise A Rollins-Smith, Emily H Le Sage

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Datasets Mentioned

BETA
PRJNA601697
AY598034

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

vegan
Geneious
PERMANOVA
R Core Team
car
megablast
R
indicspecies
Microbiome Helper
lme4

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