Frog vasoactive intestinal polypeptide and galanin: primary structures and effects on pituitary adenylate cyclase

Endocrinology
N ChartrelJ M Conlon

Abstract

Vasoactive intestinal polypeptide (VIP) and galanin were isolated in pure form from the stomach of the European green frog, Rana ridibunda. Frog VIP is identical to the previously characterized VIP from chicken and alligator. The primary structure of frog galanin contains only two amino acid substitutions (asparagine for histidine at position 23 and histidine for tyrosine at position 26) compared with porcine galanin. The data indicate that evolutionary pressure to conserve the amino acid sequence of both peptides during the evolution of amphibia to mammals has been strong. Synthetic frog VIP produced a dose-dependent increase in cAMP concentration in frog anterior pituitary fragments. The potency of the peptide (ED50 = 1.2 x 10(-6) M; mean +/- SE; n = 8) was comparable to that of porcine VIP (EC50 = 1.3 x 10(-6) M), but was approximately 10-fold less than that of frog pituitary adenylate cyclase-activating polypeptide [PACAP-(1-38); ED50 = 1.1 x 10(-7) M] in the same system. The increases in cAMP concentrations produced by maximal doses of PACAP (10(-5) M) and VIP (10(-5) M) were not additive. The data suggest that the effects of both peptides are mediated through a common PACAP-preferring receptor that is pharmacologically di...Continue Reading

Citations

May 13, 2010·BMC Evolutionary Biology·João C R CardosoDeborah M Power
Apr 21, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Michel Anctil
Feb 19, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Gen HiyamaNorio Kansaku
Jan 9, 2009·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Rosaria SciarrilloMaria De Falco
Jul 18, 2001·Microscopy Research and Technique·L YonH Vaudry
Jun 19, 2012·Clinical Epigenetics·Hiba IbrahimNeil Foster
Aug 31, 2019·The Journal of Comparative Neurology·Joel A Tripp, Andrew H Bass
May 18, 2006·The Journal of Clinical Endocrinology and Metabolism·Flavie SicardCatherine Delarue

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