Frog virus 3 ORF 53R, a putative myristoylated membrane protein, is essential for virus replication in vitro

Virology
D WhitleyV Gregory Chinchar

Abstract

Although previous work identified 12 complementation groups with possible roles in virus assembly, currently only one frog virus 3 protein, the major capsid protein (MCP), has been linked with virion formation. To identify other proteins required for assembly, we used an antisense morpholino oligonucleotide to target 53R, a putative myristoylated membrane protein, and showed that treatment resulted in marked reductions in 53R levels and a 60% drop in virus titers. Immunofluorescence assays confirmed knock down and showed that 53R was found primarily within viral assembly sites, whereas transmission electron microscopy detected fewer mature virions and, in some cells, dense granular bodies that may represent unencapsidated DNA-protein complexes. Treatment with a myristoylation inhibitor (2-hydroxymyristic acid) resulted in an 80% reduction in viral titers. Collectively, these data indicate that 53R is an essential viral protein that is required for replication in vitro and suggest it plays a critical role in virion formation.

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Citations

Dec 15, 2010·Developmental and Comparative Immunology·D S WhitleyV G Chinchar
Sep 6, 2011·Virus Research·Li-Bo HeQi-Ya Zhang
Jul 26, 2011·Virology·Jacques RobertGuangchun Chen
Jan 17, 2015·Science China. Life Sciences·QiYa Zhang, Jian-Fang Gui
Nov 13, 2014·PloS One·Jacques RobertFrancisco De Jesús Andino
Aug 9, 2016·Scientific Reports·Yongming YuanYunhan Hong
Jan 7, 2011·Journal of Virology·Chuan-Fu DongJian-Guo He
Nov 5, 2015·G3 : Genes - Genomes - Genetics·Brendan Epstein, Andrew Storfer
May 1, 2018·The Journal of General Virology·Emine OzsahinZihni Demirbag

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