From 1 H NMR-based non-targeted to LC-MS-based targeted metabolomics strategy for in-depth chemome comparisons among four Cistanche species

Journal of Pharmaceutical and Biomedical Analysis
Wenjing LiuJun Li

Abstract

The great orthogonality between 1H NMR spectroscopy and LC-MS implies that their deployments in series could offer an opportunity to gain the qualified molecular markers via comparative metabolomics, and an attempt was made here to propose an integrated strategy namely "from 1H NMR-based non-targeted to LC-MS-based targeted metabolomics". In-depth chemome comparisons of Cistanche plants, such as C. deserticola, C. salsa, C. tubulosa, and C. sinensis, that possess dramatic economic and ecological benefits for the arid regions in the northwest China attributing to their dramatic medicinal and edible values, were employed to verify the applicability. 1H NMR-based non-targeted matabolomics acted as the survey experiment to find those signals offering decisive contributions towards the species discrimination, and the signals were translated to a set of putative identities, eighteen ones in total, through matching with authentic compounds and referring to some accessible databases. Afterwards, an advanced LC-MS platform assembling reversed phase liquid chromatography, hydrophilic interaction liquid chromatography, and tailored multiple reaction monitoring, was introduced to simultaneously quantify those eighteen potential markers in ...Continue Reading

Citations

Jul 2, 2019·Chemical & Pharmaceutical Bulletin·Toshio MorikawaOsamu Muraoka
Aug 4, 2020·Chemical & Pharmaceutical Bulletin·Huibo LeiWeidong Zhang
Feb 2, 2021·Medicinal Research Reviews·Yuelin SongPengfei Tu
Mar 25, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Junsik SungDidier Merlin
Aug 30, 2021·Journal of Pharmaceutical and Biomedical Analysis·Yan CaoYuelin Song

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