From Cultured Rodent Neurons to Human Brain Tissue: Model Systems for Pharmacological and Translational Neuroscience

ACS Chemical Neuroscience
Joel Wellbourne-Wood, Jean-Yves Chatton

Abstract

To investigate the enormous complexity of the functional and pathological brain there are a number of possible experimental model systems to choose from. Depending on the research question choosing the appropriate model may not be a trivial task, and given the dynamic and intricate nature of an intact living brain several models might be needed to properly address certain questions. In this review, we aim to provide an overview of neural cell and tissue culture, reflecting on historic methodological milestones and providing a brief overview of the state-of-the-art. We additionally present an example of an effective model system pipeline, composed of dissociated mouse cultures, organotypics, acute mouse brain slices, and acute human brain slices, in that order. The sequential use of these four model systems allows a balance and progression from experimental control to human applicability, and provides a meta-model that can help validate basic research findings in a translational setting. We then conclude with a few remarks regarding the necessity of an integrated approach when performing translational and neuropharmacological studies.

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Citations

Sep 14, 2019·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Alexander R HarrisLezanne Ooi
Jun 30, 2019·Life Science Alliance·Vidhya M RaviDieter Henrik Heiland
Sep 13, 2019·Current Pharmaceutical Design·Dusica Maysinger, Jeff Ji
Dec 11, 2021·Frontiers in Neuroscience·Glaucia M AlmeidaAdriano Sebollela

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