Abstract
Determination of potential cytotoxicity is a central issue in current biocompatibility testing standards such as ISO and ASTM. Most of these tests do not assess biocompatibility of a biomaterial with regard to cell function. This study was aimed at screening a number of potential parameters that could be included in assessment of cell functional aspects of biocompatibility. Human umbilical vein endothelial cells (HUVEC) were seeded directly on titanium, NiCr alloy, CoCr alloy, PMMA, PE, PU, PVC, and silicone, or were exposed to the material extracts. Cytotoxicity was assessed for these materials through MTT conversion, crystal violet protein determination and Ki67 expression. In addition, expression of the cell adhesion molecules E-selectin, cadherin-5 and PECAM, as well as of the adhesion-associated proteins fibronectin and vinculin (focal adhesions), was determined by immunocytochemistry and western blotting. Cytotoxicity was not detected with the material extracts. Cells were able to adhere to bare metals, but not polymers. Fibronectin preadsorption resulted in adhesion and spreading also on the polymers. Cells were able to establish cell-cell contacts and focal adhesions. Western blotting, in combination with differential d...Continue Reading
Citations
Jan 20, 2005·Analytical and Bioanalytical Chemistry·E De GiglioG Spoto
Feb 27, 2007·Journal of Materials Science. Materials in Medicine·R WillumeitF Feyerabend
Aug 6, 1999·Tissue Engineering·T G van Kooten, A F von Recum
Nov 2, 2005·Tissue Engineering·Wei HeSeeram Ramakrishna
Nov 21, 2007·Journal of Biomedical Materials Research. Part a·Michael BallColin A Scotchford
Jul 11, 2007·Journal of Materials Science. Materials in Medicine·Carolina LucchesiPaulo P Joazeiro
Apr 22, 2006·Journal of Biomedical Materials Research. Part a·E A E Van TienhovenW H De Jong
Dec 17, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Karla LehleChristof Schmid
Mar 5, 2011·Journal of Materials Science. Materials in Medicine·Junping ZhengKangde Yao
Nov 7, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jennifer C Brennan, Donald E Tillitt
Dec 13, 2018·Biomacromolecules·Johanna TranJulien Nicolas
Jun 20, 2017·Analytical Chemistry·Gert Ij SalentijnElisabeth Verpoorte
Jan 16, 2008·Biomaterials·Eoin D O'CearbhaillValerie Barron