From DMEK to Corneal Endothelial Cell Therapy: Technical and Biological Aspects

Journal of Ophthalmology
Raffaele NuzziFederico Tridico

Abstract

The main treatment available for restoration of the corneal endothelium is keratoplasty and DMEK provides faster visual recovery and better postoperative visual acuity when compared to DSAEK. However, the technical challenges related to this technique and the steep technical learning curve seem to prevent the overcoming of DSAEK in favor of DMEK. Furthermore, the outcome of lamellar keratoplasty techniques is influenced by problems related to corneal grafting tissue availability, management, and quality. On the other hand, improvements in the field of cell engineering have opened the way for the use of stem cells-derived corneal endothelial cells with regenerative intent. In this overview, latest findings in endothelial cell engineering are reported, and perspectives of clinical application of mesenchymal stem cells for corneal endothelial replacement and regeneration are evaluated.

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Citations

Oct 9, 2019·International Journal of Molecular Sciences·Ayidah AlghuwainemBatla Alsowayan
Jan 6, 2019·Journal of Biomedical Science·Shuangling ChenYingting Zhu
Jan 26, 2021·Experimental Eye Research·Pierre Antoine FayeYohan Benayoun
Aug 10, 2021·Tissue & Cell·Mohsen GhiasiHossein Aghamollaei

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Methods Mentioned

BETA
scanning electron microscopy

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