From electron crystallography of 2D crystals to MicroED of 3D crystals.

Current Opinion in Colloid & Interface Science
Michael W Martynowycz, Tamir Gonen

Abstract

Electron crystallography is widespread in material science applications, but for biological samples its use has been restricted to a handful of examples where two-dimensional (2D) crystals or helical samples were studied either by electron diffraction and/or imaging. Electron crystallography in cryoEM, was developed in the mid-1970s and used to solve the structure of several membrane proteins and some soluble proteins. In 2013, a new method for cryoEM was unveiled and named Micro-crystal Electron Diffraction, or MicroED, which is essentially three-dimensional (3D) electron crystallography of microscopic crystals. This method uses truly 3D crystals, that are about a billion times smaller than those typically used for X-ray crystallography, for electron diffraction studies. There are several important differences and some similarities between electron crystallography of 2D crystals and MicroED. In this review, we describe the development of these techniques, their similarities and differences, and offer our opinion of future directions in both fields.

Citations

Sep 2, 2018·Proceedings of the National Academy of Sciences of the United States of America·Helen M E DuyvesteynDavid I Stuart
Sep 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krishna P KhakurelJakob Andreasson
Nov 17, 2020·Magnetic Resonance in Chemistry : MRC·Mikhail Elyashberg, Dimitris Argyropoulos
Jan 20, 2021·Chemical Reviews·Thomas BuhseJean-Claude Micheau
Jun 22, 2021·Annual Review of Biochemistry·Xuelang MuTamir Gonen
Dec 18, 2018·ACS Central Science·Christopher G JonesTamir Gonen

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