From extensive clone libraries to comprehensive DNA arrays for the efficient and simultaneous detection and identification of orchid mycorrhizal fungi

Journal of Microbiological Methods
Bart LievensHans Jacquemyn

Abstract

A DNA array was developed from extensive clone library sequence data sets for the assessment of dominant members of mycorrhizal fungi that associate with terrestrial orchid species. As a-proof-of-concept, the array was developed for the basidiomycetous mycorrhizal partners from three closely related perennial Orchis species, including Orchis anthropophora, O. militaris and O. purpurea. Based on internal transcribed spacer regions, oligonucleotides were developed for seven operational taxonomic units (OTUs; defined as groups of sequences sharing at least 97% sequence similarity), corresponding to members of the Tulasnellaceae family. In order to cover a broader spectrum of tulasnelloid fungi, oligonucleotides were as well developed for two subsets of closely related OTUs. The array was evaluated using multiple primer pairs. In addition, hybridization results were validated by recovery and sequencing of the hybridized amplicons as well as by hybridizing reference DNA samples. Considering the unlimited expansion possibilities of DNA arrays to include specific detector oligonucleotides for other and more microorganisms, the method described here has the major advantage that it provides a powerful, rapid and cost-effective way for t...Continue Reading

References

Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·M SchenaR W Davis
Jan 3, 2001·Applied and Environmental Microbiology·A G SpeksnijderH J Laanbroek
Jun 1, 2002·Applied and Environmental Microbiology·John DunbarCheryl R Kuske
Sep 6, 2003·Science·Christopher W SchadtSteven K Schmidt
Jul 9, 2004·Applied and Environmental Microbiology·Adelaide E WarsenDouglas R Call
Jul 31, 2004·Annual Review of Phytopathology·Mark Mazzola
Sep 10, 2005·Applied and Environmental Microbiology·Heath E O'BrienRytas Vilgalys
Oct 20, 2005·Environmental Microbiology·Bart LievensBart P H J Thomma
Jan 27, 2006·FEMS Microbiology Letters·Bart LievensBart P H J Thomma
May 9, 2006·Ecology·Melissa K McCormickBrendan Hodkinson
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 5, 2007·Evolution; International Journal of Organic Evolution·Richard P SheffersonYung-I Lee
Sep 25, 2007·The New Phytologist·Hans JacquemynThorsten Wiegand
Nov 29, 2007·The ISME Journal·Luiz F W RoeschEric W Triplett
Feb 12, 2008·Trends in Genetics : TIG·Mihai Pop, Steven L Salzberg
Nov 5, 2008·Microbial Ecology·Ariana KubartováThierry Beguiristain
Nov 6, 2008·Current Opinion in Biotechnology·Erick Cardenas, James M Tiedje
Mar 11, 2009·Mycologia·Joke Pannecoucque, Monica Höfte
Feb 1, 2008·American Journal of Botany·Richard P SheffersonKadri Tali

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Citations

Apr 5, 2011·The American Naturalist·Richard J WatermanAnton Pauw
Oct 1, 2011·The New Phytologist·Hans JacquemynThorsten Wiegand
Apr 5, 2011·Journal of Microbiological Methods·Evans N NjambereNing Zhang
May 28, 2011·American Journal of Botany·Bertrand SchatzMarc-André Selosse
Oct 4, 2012·American Journal of Botany·Bruno Cachapa BailaroteHans Jacquemyn

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