From individual to collective 3D cancer dissemination: roles of collagen concentration and TGF-β.

Scientific Reports
J PlouJ M García-Aznar

Abstract

Cancer cells have the ability to migrate from the primary (original) site to other places in the body. The extracellular matrix affects cancer cell migratory capacity and has been correlated with tissue-specific spreading patterns. However, how the matrix orchestrates these behaviors remains unclear. Here, we investigated how both higher collagen concentrations and TGF-β regulate the formation of H1299 cell (a non-small cell lung cancer cell line) spheroids within 3D collagen-based matrices and promote cancer cell invasive capacity. We show that at low collagen concentrations, tumor cells move individually and have moderate invasive capacity, whereas when the collagen concentration is increased, the formation of cell clusters is promoted. In addition, when the concentration of TGF-β in the microenvironment is lower, most of the clusters are aggregates of cancer cells with a spheroid-like morphology and poor migratory capacity. In contrast, higher concentrations of TGF-β induced the formation of clusters with a notably higher invasive capacity, resulting in clear strand-like collective cell migration. Our results show that the concentration of the extracellular matrix is a key regulator of the formation of tumor clusters that af...Continue Reading

References

Jul 11, 1998·Molecular Biology of the Cell·A ErlebacherR Derynck
Mar 24, 2004·Current Opinion in Cell Biology·Peter Friedl
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Aug 4, 2007·American Journal of Respiratory Cell and Molecular Biology·Yasushi ShintaniMargaret J Wheelock
Jul 30, 2008·Cell·Joan Massagué
Nov 15, 2008·Clinical Journal of the American Society of Nephrology : CJASN·Bart Clarke
Dec 20, 2008·Journal of Microscopy·Andrew M SteinLeonard M Sander
Jan 24, 2009·Nature Reviews. Cancer·Darci T ButcherValerie M Weaver
Jan 16, 2010·EMBO Reports·Coert Margadant, Arnoud Sonnenberg
Aug 12, 2010·Bone·Patricia Juárez, Theresa A Guise
Sep 9, 2010·Cell Communication and Signaling : CCS·Jan BrábekBen Fabry
Nov 19, 2010·PloS One·Nazanin S RuppenderScott A Guelcher
Sep 16, 2011·Cold Spring Harbor Perspectives in Biology·Pengfei LuZena Werb
Nov 29, 2011·Cell·Peter Friedl, Stephanie Alexander
Jan 13, 2012·Molecular Biology of the Cell·Jennifer L LeightChristopher S Chen
Aug 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kim-Vy Nguyen-NgocAndrew J Ewald
Jan 25, 2013·Cancer Research·Thomas R CoxJanine T Erler
Jan 1, 2012·BoneKEy Reports·Jeroen T BuijsTheresa A Guise
Dec 3, 2013·Cell Reports·Daisuke HoshinoAlissa M Weaver
Jan 11, 2014·Journal of Biomedical Optics·Jeremy S BredfeldtKevin W Eliceiri
Jan 22, 2014·The Journal of Cell Biology·Nadine TarantinoEmmanuel Laplantine
Apr 9, 2014·Nature Medicine·Victoria Seewaldt
Apr 12, 2014·Biochimica Et Biophysica Acta·Anna HaegerPeter Friedl
Jul 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thomas R Cox, Janine T Erler
Aug 8, 2014·Journal of the Royal Society, Interface·Wei SunNicholas Agung Kurniawan
Nov 12, 2014·PLoS Biology·Bojana GligorijevicJohn Condeelis
Dec 30, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·L E BarneyS R Peyton
May 28, 2015·Annals of Biomedical Engineering·O Moreno-ArotzenaJ M García-Aznar
Jul 26, 2015·Cancer Discovery·Ravikanth Maddipati, Ben Z Stanger
Aug 25, 2015·Cell Adhesion & Migration·Marina Krause, Katarina Wolf
Nov 1, 2015·Advanced Drug Delivery Reviews·Hinke A B MulthauptJohn R Couchman
Nov 12, 2015·Journal of Biomechanics·Cristina Del AmoJosé Manuel García-Aznar
Jan 23, 2016·Nature·Joan Massagué, Anna C Obenauf

❮ Previous
Next ❯

Citations

Oct 20, 2018·Science·Igor Adameyko
Mar 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Susan E LeggettIan Y Wong
Oct 21, 2020·Translational Oncology·Jia-Shun WuXin-Hua Liang
Feb 27, 2021·PLoS Computational Biology·Inês G Gonçalves, Jose Manuel Garcia-Aznar
Mar 2, 2021·Advanced Science·Barbara Blanco-FernandezJoão F Mano
Jun 3, 2021·Clinical & Experimental Metastasis·Jenna A MosierCynthia A Reinhart-King
Jul 25, 2021·International Journal of Molecular Sciences·Jeff Yat-Fai ChungPatrick Ming-Kuen Tang

❮ Previous
Next ❯

Software Mentioned

MATLAB

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.