From Inflammation to Cutaneous Repair: Topical Application of Lupeol Improves Skin Wound Healing in Rats by Modulating the Cytokine Levels, NF-κB, Ki-67, Growth Factor Expression, and Distribution of Collagen Fibers.

International Journal of Molecular Sciences
Fernando Pereira BeserraCláudia Helena Pellizzon

Abstract

Skin wound healing is a highly complex event that involves different mediators at the cellular and molecular level. Lupeol has been reported to possess different biological activities, such as anti-inflammatory, antioxidant, antidiabetic, and in vitro wound healing properties, which motivated us to proceed with in vivo studies. We aimed to investigate the wound healing effect of lupeol-based cream for 3, 7, and 14 days. Wound excisions were induced on the thoraco-lumbar region of rats and topically treated immediately after injury induction. Macroscopic, histopathological, and immunohistochemical analyses were performed. Cytokine levels were measured by ELISA and gene expression was evaluated by real-time RT-qPCR. Our results showed a strong wound-healing effect of lupeol-based cream after 7 and 14 days. Lupeol treatment caused a reduction in proinflammatory cytokines (TNF-a, IL-1β, and IL-6) and gene and protein NF-κB expression, and positively altered IL-10 levels, showing anti-inflammatory effects in the three treatment periods. Lupeol treatment showed involvement in the proliferative phase by stimulating the formation of new blood vessels, increasing the immunostaining of Ki-67 and gene expression, and immunolabeling of vas...Continue Reading

References

Nov 5, 1999·Biochemical and Biophysical Research Communications·Y SatoT Kondo
Mar 13, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M SaleemS Sultana
Mar 15, 2003·Clinics in Plastic Surgery·David G Greenhalgh
Jul 5, 2003·Physiological Reviews·Sabine Werner, Richard Grose
Jan 7, 2005·Nature Medicine·Michael Karin
Oct 12, 2005·Life Sciences·Varatharajan SudhaharPalaninathan Varalakshmi
Jan 22, 2008·Journal of Cellular and Molecular Medicine·W Wagner, M Wehrmann
Jan 29, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·B G HarishH M Kumara Swamy
Jan 30, 2008·Current Protocols in Cell Biology·Randall I Krohn
Mar 8, 2008·Pharmacological Reviews·Nader G Abraham, Attallah Kappas
Sep 24, 2010·PloS One·Rafael Henrique NóbregaRüdiger W Schulz
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Oct 19, 2011·The Journal of Surgical Research·Riaz AghaDennis P Orgill
Dec 20, 2011·Journal of the American Academy of Dermatology·Christos ProfyrisIsabel Do Vale
Jul 7, 2012·The Journal of Pharmacology and Experimental Therapeutics·Christophe MorinJosé Courty
Jan 29, 2013·Experimental Biology and Medicine·Ralph Gruppi ThoméRosy Iara Maciel Azambuja Ribeiro
Oct 23, 2013·Clinical Therapeutics·Arthur TallisUNKNOWN Collagenase Diabetic Foot Ulcer Study Group
Mar 4, 2014·Journal of Ethnopharmacology·Maria Liduína Maia de OliveiraDiana Célia Sousa Nunes-Pinheiro
Sep 6, 2014·Cold Spring Harbor Perspectives in Biology·Toshio TanakaTadamitsu Kishimoto
Oct 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria C C CanessoLucíola S Barcelos
Dec 4, 2014·Molecular and Clinical Oncology·Inoru YokoeYoshiharu Okamoto
Mar 18, 2015·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Ümit GönüllüZeynep Aydoğmuş
Jul 1, 2015·BMC Complementary and Alternative Medicine·Jamila Alessandra PeriniDaniel Escorsim Machado
Jul 4, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Haroon BadshahMyeong Ok Kim
Aug 25, 2016·Plastic and Reconstructive Surgery·Linsey E LindleyMarjana Tomic-Canic
Sep 21, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Lucas Fernando Sérgio GushikenCláudia Helena Pellizzon
Nov 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fernando Pereira BeserraChristopher John Jackson

❮ Previous
Next ❯

Citations

Dec 15, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kai LiuJia Luo
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ramona Daniela Pârvănescu PanăCristina Adriana Dehelean

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Reverse
PCR

Software Mentioned

Adobe Photoshop
AVSoftBioView
CellSens Standard
GraphPad Prism

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.