From PCBs to highly toxic metabolites by the biphenyl pathway

Environmental Microbiology
Beatriz CámaraMichael Seeger

Abstract

The degradation of polychlorobiphenyls (PCBs) by diverse bacteria, including Burkholderia sp. LB400, is incomplete with a concomitant accumulation of metabolic intermediates. In this study, the toxicity of diverse (chloro)biphenyls and of their biotransformation into the first two metabolic intermediates of the biphenyl pathway, were determined for the model bacterium Escherichia coli. Recombinant E. coli strains expressing different subsets of bph genes of strain LB400 accumulated metabolic intermediates from (chloro)biphenyls. During biotransformation of these compounds into metabolic intermediates, the viability and metabolic kinetics were determined. The toxicity of biotransformation of (chloro)biphenyls into different metabolic intermediates of (chloro)biphenyls varied. Dihydrodiols and dihydroxybiphenyls are very toxic metabolites for bacteria even after short incubation times, affecting the cell viability much more than (chloro)biphenyls. When bacteria transformed 2-CB into dihydrodiol or dihydroxybiphenyl, a great decrease of intact cells and abundant cell lysis was observed by transmission electronic microscopy. Cell viability of Burkholderia sp. LB400 and of E. coli exposed directly to 2,3-dihydroxybiphenyl decreased ...Continue Reading

References

Aug 1, 1979·Applied and Environmental Microbiology·K FurukawaA Kamibayashi
May 5, 1991·Journal of Molecular Biology·F W Studier
Jan 1, 1990·Critical Reviews in Toxicology·E M SilberhornL W Robertson
Jan 1, 1973·Canadian Journal of Microbiology·M Ahmed, D D Focht
Jan 23, 1973·Biochemical and Biophysical Research Communications·D T GibsonV M Kobal
Sep 1, 1982·Applied and Environmental Microbiology·K Furukawa, A M Chakrabarty
Nov 15, 1996·Journal of Biotechnology·E RosenbergE Z Ron
Feb 28, 2001·Environmental Microbiology·H LünsdorfK N Timmis
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Sep 6, 2003·Applied and Environmental Microbiology·Michael SeegerSilvia Sepúlveda-Boza
Mar 1, 1984·Applied and Environmental Microbiology·I BartelsW Reineke

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Citations

Jun 23, 2006·Analytical and Bioanalytical Chemistry·Jessika FelicianoSylvia Daunert
Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Aug 25, 2012·Biotechnology Letters·Rouzbeh TehraniBenoit Van Aken
Dec 5, 2012·Environmental Science and Pollution Research International·Simona Di GregorioRoberto Lorenzi
May 15, 2009·International Journal of Environmental Research and Public Health·Osnat SegevAsher Brenner
Apr 3, 2014·Applied Microbiology and Biotechnology·Sebastián FuentesMichael Seeger
May 3, 2013·Environmental Science and Pollution Research International·Rouzbeh Tehrani, Benoit Van Aken
Apr 17, 2013·Environmental Science and Pollution Research International·Rouzbeh TehraniBenoit Van Aken
Jan 17, 2013·Environmental Science & Technology·Guangshu ZhaiJerald L Schnoor
Dec 7, 2011·Enzyme and Microbial Technology·Bernardita L PonceMichael Seeger
Mar 3, 2009·Current Opinion in Biotechnology·Michel SylvestreMartina Mackova
Jan 18, 2006·Biotechnology Advances·Francisco P ChávezCarlos A Jerez
Mar 22, 2007·FEMS Microbiology Ecology·Felipe GordilloCarlos A Jerez
May 26, 2017·Environmental Science & Technology·Srishty SubramanianBenoit Van Aken
May 11, 2017·Environmental Science and Pollution Research International·Jitendra K SharmaAsha A Juwarkar
Jan 29, 2019·International Journal of Systematic and Evolutionary Microbiology·Fernanda ClaveríasBeatriz Camara
Nov 18, 2020·Journal of Hazardous Materials·Tatyana I GorbunovaOleg N Chupakhin

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