From plasma membrane to cytoskeleton: a novel function for semaphorin 6A

Molecular Cancer Therapeutics
Silvia PrisleiCristiano Ferlini

Abstract

Class III beta-tubulin (TUBB3) overexpression has been reported in ovary, lung, breast, and gastric cancer patients. Currently, no clinical drugs are available for a specific targeting of TUBB3, whereas the investigational drug IDN5390 specifically interacts with TUBB3. To gain insight into the pathways leading to TUBB3 up-regulation, we did a human genome microarray analysis in A2780 cells made resistant to IDN5390 to identify selected pathways specifically disrupted in resistant cells. Using this approach, we discovered that semaphorin 6A (SEMA6A) is down-regulated not only in IDN5390-resistant cells but also in cells made resistant to cisplatin, topotecan, and doxorubicin, whereas no changes were noticed in paclitaxel- and gemcitabine-resistant cells. Acute treatment with IDN5390 was able to down-regulate SEMA6A in cells unselected for drug resistance. TUBB3 expression was assessed in A2780 clones with stable overexpression of SEMA6A and in a panel of clones in which silencing of the protein was obtained. Quantitative PCR was then used to check the modulation of SEMA6A as well as to assess the expression of TUBB3. TUBB3 was increased (median value, 5.4) and reduced (median value, 0.47) in cells with overexpression and silenc...Continue Reading

References

Jan 1, 1997·Molecular and Cellular Neurosciences·F EckhardtM C Fishman
Aug 29, 2002·Nature Cell Biology·Silvia GiordanoPaolo M Comoglio
Jul 2, 2003·Nature Cell Biology·Olga C RodriguezClare M Waterman-Storer
Oct 25, 2003·Oncogene·George A OrrSusan Band Horwitz
Jun 5, 2004·Genomics·Philippe ColletMichel Dauça
Nov 16, 2004·Nature Cell Biology·Toshihiko ToyofukuHitoshi Kikutani
Dec 8, 2004·Current Opinion in Structural Biology·Ermanno GherardiE Yvonne Jones
Apr 14, 2005·Molecular Cancer Therapeutics·Christopher P LandowskiGordon L Amidon
Jun 1, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A ParadisoV Lorusso
Jul 15, 2005·Cell Death and Differentiation·A ChédotalC Moreau-Fauvarque
Aug 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pascal SèveCharles Dumontet
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Robert P KrugerKun-Liang Guan
Apr 6, 2006·Genome Biology·Umar Yazdani, Jonathan R Terman
May 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gabriella FerrandinaCristiano Ferlini
Oct 5, 2006·Journal of the National Cancer Institute·Nicole M VerrillsMaria Kavallaris

❮ Previous
Next ❯

Citations

Sep 2, 2010·PloS One·Regina Berretta, Pablo Moscato
Feb 15, 2012·Gene Expression Patterns : GEP·A M EbertS McFarlane
May 28, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jiaxin ZhaoPeng Liang
Apr 27, 2011·Journal of Cellular Physiology·Marta De DonatoCristiano Ferlini
Apr 4, 2015·Gene·Marisa MarianiCristiano Ferlini
Sep 12, 2012·Biochemical Pharmacology·Simona MozzettiCristiano Ferlini
Mar 31, 2009·Biochimica Et Biophysica Acta·J Wolff
Oct 1, 2010·Developmental Biology·Carl M GayJesús Torres-Vázquez
Jul 6, 2014·Frontiers in Oncology·Amelia L ParkerJoshua A McCarroll
Mar 23, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marisa MarianiCristiano Ferlini
Dec 18, 2008·Molecular Cancer·Massimiliano MonticonePatrizio Castagnola

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.