From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing

Genes & Development
Yao Wan, Anita K Hopper

Abstract

The mitochondrial cytoplasmic surface serves as a processing site for numerous RNAs from budding yeast to metazoans. We report that budding yeast mitochondrial outer membrane (MOM) proteins that are subunits of the translocase of the outer mitochondrial membrane (Tom70 and Tom 22) and sorting and assembly machinery (Sam37) are required for efficient pretransfer RNA (pre-tRNA) splicing. Defective pre-tRNA splicing in MOM mutants is due not to loss of respiratory metabolism but instead inefficient targeting/tethering of tRNA splicing endonuclease (SEN) subunits to mitochondria. Schizosaccharomyces pombe SEN subunits also localize to mitochondria, and Tom70 is required for this localization and pre-tRNA splicing. Thus, the role of MOM protein in targeting/tethering SEN subunits to mitochondria has been conserved for >500 million years.

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Citations

Mar 8, 2019·Frontiers in Genetics·Anita K Hopper, Regina T Nostramo
Sep 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Hugo C Medina-MunozMarvin Wickens
Oct 7, 2020·International Journal of Molecular Sciences·Sebastian Kreimendahl, Joachim Rassow
Oct 20, 2020·Nucleic Acids Research·Regina T Nostramo, Anita K Hopper
Oct 31, 2020·BMC Biology·Sebastian KreimendahlJoachim Rassow
Mar 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jennifer E HurtigAmbro van Hoof
Apr 22, 2021·Science China. Life Sciences·Jing LiRu-Juan Liu
May 1, 2021·The FEBS Journal·Nayef JarrousAravind Ramanathan

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