From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli

Journal of Proteome Research
Li-na WuXuanxian Peng

Abstract

Proteomic technology is very powerful in identification of differentially expressed proteins. However, how to identify key proteins and distinguish them from others has been a question to be solved in functional proteomics. Utilizing 2-D gel based proteomic approach, we identified 11 differentially expressed outer membrane (OM) proteins involved in E. coli's response to pH change. The protein expression changes were validated by Western blotting. The function and roles of the differentially expressed proteins were further characterized using genetically modified strains with gene deletion of these altered OM proteins and gene complementation or overexpression approach. Among the differentially expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins. Out of these OM proteins, TolC, OmpC, OmpX, and LamB may play critical roles in pH-regulation in E. coli. Using death-rescuing assay developed in house, we found that OmpC, LamB, FadL, OmpX, OmpW, and OmpF, LamB, FadL, and OmpW functioned in a TolC-independent pathway, whereas OmpF, Tsx and OmpC, OmpX, and Tsx might share the same pathway with TolC at the extreme acid or base condition....Continue Reading

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Citations

Oct 26, 2010·Current Microbiology·Dan-Feng ZhangXuan-Xian Peng
Mar 29, 2011·Foodborne Pathogens and Disease·Rebecca M Goulter-ThorsenGary A Dykes
Jun 9, 2009·Microbial Cell Factories·Riccardo VillaPietro Gatti-Lafranconi
Dec 13, 2018·Applied Microbiology and Biotechnology·María Elisa PavanM Julia Pettinari

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