From sample preparation to NMR-based metabolic profiling in food commodities: The case of table olives.

Phytochemical Analysis : PCA
Stavros BeteinakisMaria Halabalaki

Abstract

Nuclear magnetic resonance (NMR)-based metabolic profiling has been widely used in food and plant sciences. Despite its simplicity and inherent reproducibility, the determination of the appropriate pre-processing procedures greatly affects the obtained metabolic profile. The current study represents a detailed guide of use for untargeted NMR-based metabolic profiling of table olives (Olea europaea L.). Greek Kalamon table olives from different geographical origins were selected as reference materials. Differently treated samples were extracted using different solvents and/or solvent systems. Chemical profiles were evaluated with high-performance thin layer chromatography (HPTLC). Different deuterated solvents and sample concentrations were evaluated for the recording of optimal quality spectra. The methanol extract of freeze-dried table olives was found to contain the most representative secondary metabolites, in higher concentrations, as well. The optimal deuterated solvent for the NMR analysis was methanol-d4 , while final sample concentration should be within the range of 10 to 15 mg/mL. Multivariate data analysis was also used to estimate and confirm the variation and clustering caused by different characteristics of the sa...Continue Reading

References

May 26, 2004·Environmental Health Perspectives·Katja Dettmer, Bruce D Hammock
Dec 5, 2009·Journal of Agricultural and Food Chemistry·Evagelia ZoidouAlexios-Leandros Skaltsounis
Mar 5, 2011·Mass Spectrometry Reviews·Miguel HerreroAlejandro Cifuentes
Oct 16, 2012·Analytica Chimica Acta·Agnieszka SmolinskaSybren S Wijmenga
Feb 6, 2014·Phytochemical Analysis : PCA·Maria HalabalakiJean-Luc Wolfender
Jul 21, 2015·Food Chemistry·Marc SpiteriDouglas N Rutledge
Sep 14, 2015·Journal of Chromatography. a·Monica Gallo, Pasquale Ferranti
Jun 30, 2019·Metabolites·Abdul-Hamid EmwasDavid S Wishart
Jul 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natasa P KalogiouriNikolaos S Thomaidis
Jul 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stavros BeteinakisMaria Halabalaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Ana Paula PereiraM Letícia Estevinho
Current Biology : CB
Anne Knowlton
Journal of Nutritional Science
Janete RochaOlívia Pinho
Journal of Agricultural and Food Chemistry
Suthawan Charoenprasert, Alyson Mitchell
© 2021 Meta ULC. All rights reserved