From the Cover: The dynamics of genomic-length DNA molecules in 100-nm channels

Proceedings of the National Academy of Sciences of the United States of America
Jonas O TegenfeldtRobert H Austin

Abstract

We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of lambda DNA multimers with a 130-nm SD in 1 min.

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Citations

Mar 15, 2008·Analytical and Bioanalytical Chemistry·Nicholas DouvilleShuichi Takayama
Apr 23, 2008·Analytical and Bioanalytical Chemistry·Liza LamHiroyuki Noji
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