Frosted Slides Decorated with Silica Nanowires for Detecting Circulating Tumor Cells from Prostate Cancer Patients

ACS Applied Materials & Interfaces
Haijun CuiShutao Wang

Abstract

Developing low-cost and highly efficient nanobiochips are important for liquid biopsies, real-time monitoring, and precision medicine. By in situ growth of silica nanowires on a commercial frosted slide, we develop a biochip for effective circulating tumor cells (CTCs) detection after modifying epithelial cell adhesion molecule antibody (anti-EpCAM). The biochip shows the specificity and high capture efficiency of 85.4 ± 8.3% for prostate cancer cell line (PC-3). The microsized frosted slides and silica nanowires allow enhanced efficiency in capture EpCAM positive cells by synergistic topographic interactions. And the capture efficiency of biochip increased with the increase of silica nanowires length on frosted slide. The biochip shows that micro/nanocomposite structures improve the capture efficiency of PC-3 more than 70% toward plain slide. Furthermore, the nanobiochip has been successfully applied to identify CTCs from whole blood specimens of prostate cancer patients. Thus, this frosted slide-based biochip may provide a cheap and effective way of clinical monitoring of CTCs.

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Citations

Jan 17, 2020·Advanced Materials·Stuart G HigginsMolly M Stevens
Jun 14, 2020·Journal of Functional Biomaterials·Giuseppe ArrabitoBruno Pignataro
Apr 16, 2020·Physical Review. E·Hang Zang, Xinlei Li
Feb 3, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Xiao HeShutao Wang
Nov 27, 2020·Advanced Science·Wenning JiangLingyun Jia
Apr 30, 2020·Analytical Chemistry·Jiaxiang YinXingyu Jiang

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