Frozen-stored granulocytes can be used for an immunofluorescence test to detect granulocyte antibodies

Transfusion
M NishimuraT Juji

Abstract

Donor- and/or recipient-derived granulocyte antibodies are considered to be the main cause of transfusion-related acute lung injury (TRALI), neutropenia, and febrile transfusion reactions. Several types of tests are performed to detect antibodies in donated blood and/or the serum of a transfusion recipient. Because granulocytes cannot endure the freezing-thawing process, they cannot be stored in liquid nitrogen (LN2). Therefore, testing is time-consuming, because freshly prepared granulocytes are needed for each testing. An attempt has been made to develop a method that uses granulocytes stored in LN2 for the granulocyte immunofluorescence test (GIFT). Freshly prepared granulocytes were suspended in a solution of 90-percent fetal bovine serum (FBS) plus 10-percent DMSO and then frozen and stored in LN2. In the case of GIFT, frozen-stored granulocytes were rapidly thawed, washed, and fixed with 1-percent paraformaldehyde (PFA) and then treated with MoAbs or serum containing antibodies that were reactive to granulocytes. After staining of granulocytes with FITC or PE, FACS analysis was performed. A comparison of FACS profiles of freshly prepared granulocytes stained with MoAbs or serum with FACS profiles of frozen-thawed-fixed gr...Continue Reading

Citations


❮ Previous
Next ❯