PMID: 1201464Nov 24, 1975Paper

Frozen thin-sections of rapidly forming bone: bone cell ultrastructure

Calcified Tissue Research
C V Gay, H Schraer

Abstract

Frozen thin-sections of fresh medullary bone, which had been calcifying for 2 to 4 days, were prepared and examined in the electron microscope. The bone was obtained from male Japanese quail treated with estradiol valerate. Large numbers of 200-800 A electron dense granules, which consisted of 50-75 A subparticles, were seen within mitochondria. A population of electron-dense particles slightly smaller than ribosomes was also observed. Most granules were not present after conventional fixation, dehydration, embedding, sectioning and staining. Oother structures which were visible, also on the basis of their intrinsic electron density, were nuclei with regions which resembled condensed chromatin, ribosomes and rough endoplasmic reticulum. By the use of osmium tetroxide vapor staining, inner and outer membranes and cristae of mitochondria were delineated. Other membranous components were not detected. Since very little loss or dislocation of cell components can occur during the preparation of frozen thin-sections, the micrographs obtained may be a more accurate representation of cell ultrastructure.

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Citations

Sep 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M D McKeeM J Glimcher
Dec 8, 1978·Calcified Tissue Research·E H Burger, J L Matthews
Oct 1, 2013·Calcified Tissue International·Sidney OmelonAntonio Nanci
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Julia MahamidLia Addadi
Sep 1, 1977·Journal of Oral Pathology·G L Becker
Jan 1, 1987·Mayo Clinic Proceedings·E A WilliamsP J Kelly
May 1, 1977·Journal of Ultrastructure Research·W J LandisM J Glimcher
May 20, 2015·Bone·Irving M ShapiroMakarand V Risbud
May 20, 2008·Bone·Adam M ZahmChristopher S Adams
May 18, 2011·Cells, Tissues, Organs·Julia MahamidSteve Weiner
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Ozawa, T Yamamoto
Apr 1, 1977·Journal of Ultrastructure Research·W J LandisM J Glimcher
Feb 1, 1980·Journal of Ultrastructure Research·W J LandisM J Glimcher
Jan 30, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sharmistha NaskarBikramjit Basu
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hidehiro Ozawa, Toshio Yamamoto
May 1, 2020·Membranes·Jerzy J JasielecAndrzej Lewenstam
Jan 1, 1983·Metabolic Bone Disease & Related Research·D C MorrisH C Anderson

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