Abstract
The aim of this study was to investigate the effects that prebiotic and candidates for prebiotics on Clostridium difficile strains to adhere to various human epithelial cell lines and to compare the adhesive properties of specific C. difficile strains. We also sought to examine the effect of different concentrations of fructooligosaccharides and mannose on the formation of biofilms by C. difficile strains. The influence of cellobiose, fructooligosaccharides, inulin, mannose, and raffinose on the adherence properties of various C. difficile strains, including motile 630, non-motile M120, and 10 clinical motile ribotype 027 strains, to non-mucous secreting HT-29, mucous secreting HT-29 MXT, and CCD 841 CoN cells lines. The most effective prebiotics were used in biofilm formation assays. We demonstrated that all C. difficile strains adhered to all cell lines. However, the C. difficile M120 non-motile strain was statistically more likely to adhere to all three cell lines (CFU median, 40) compared to the motile strains (CFU median, 3; p < 0.001). Furthermore, among the carbohydrates examined, only fructooligosaccharides and mannose were found to significantly decrease adhesion (p < 0.001) of C. difficile strains. Alternatively, usin...Continue Reading
References
Oct 1, 1994·Infection and Immunity·T KarjalainenP Bourlioux
Sep 1, 1994·Journal of Medical Microbiology·A PantostiF D'Ambrosio
Nov 1, 1995·Critical Reviews in Food Science and Nutrition·J van LooG Smits
Jan 16, 1999·Journal of Clinical Microbiology·S L StubbsB I Duerden
Jul 8, 1999·The Journal of Nutrition·K R Niness
Oct 3, 2003·FEMS Immunology and Medical Microbiology·Itzhak OfekNathan Sharon
Jul 13, 2006·BMC Microbiology·Roberta CretiJohannes Huebner
Sep 20, 2006·Infection and Immunity·Kari ShoafRobert W Hutkins
Mar 28, 2009·Journal of Pediatric Gastroenterology and Nutrition·Mitchell B Cohen
Jun 17, 2009·Nature Reviews. Microbiology·Maja RupnikDale N Gerding
Sep 15, 2009·Journal of Bacteriology·Susan M TwineSusan M Logan
Sep 29, 2009·Genome Biology·Richard A StablerBrendan W Wren
Dec 23, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mark MillerSharon Kelly
Jun 8, 2012·FEBS Letters·Mirja HartmannClaudia Röhl
Aug 2, 2012·Infection and Immunity·Annie AubrySusan M Logan
Feb 6, 2014·PloS One·Ekaterina G SemenyukAdam Driks
Aug 8, 2014·FEMS Microbiology Ecology·Gabriella C van ZantenMogens Jakobsen
Feb 19, 2015·Foodborne Pathogens and Disease·Shanshan WangXiaolu Jiang
Feb 24, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J FreemanUNKNOWN Pan-European Longitudinal Surveillance of Antibiotic Resistance among Prevalent Clostridium difficile Ribotypes' Study Group
Nov 5, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Hanna PituchUNKNOWN Polish Clostridium difficile Study Group
Mar 29, 2016·Anaerobe·M AltamimiR A Rastall
Jun 15, 2017·Nature Reviews. Gastroenterology & Hepatology·Glenn R GibsonGregor Reid
Jan 9, 2018·Nature·J CollinsR A Britton
May 8, 2018·Anaerobe·Adam P Roberts, Wiep Klaas Smits
Jun 24, 2018·Anaerobe·Kaarel AdambergTiina Alamäe
Jul 7, 2018·NPJ Biofilms and Microbiomes·Lydia C PowellDavid W Thomas
Sep 21, 2018·Cell Reports·Vandana SharmaHudson H Freeze
Nov 22, 2018·Frontiers in Microbiology·Ursula Waack, Tracy L Nicholson
Dec 12, 2018·Microbiology Resource Announcements·Anand KumarAlex Rodriguez-Palacios
Citations
Mar 7, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Dorota WultańskaHanna Pituch
Aug 8, 2020·Allergy·Charlotte A AlemaoPhilip M Hansbro
Oct 22, 2020·Open Biology·Arturo Aguilar-RojasNancy Guillen
Nov 6, 2020·Food & Function·Chunli KongRenate Akkerman
Mar 7, 2021·Toxins·Mostafa AsadpoorSaskia Braber
Jul 22, 2021·Food & Function·Chunli KongPaul de Vos
Jul 28, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Julio E Arce MirandaMa Gabriela Paraje