Fructooligosaccharides and mannose affect Clostridium difficile adhesion and biofilm formation in a concentration-dependent manner

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Michał PiotrowskiHanna Pituch

Abstract

The aim of this study was to investigate the effects that prebiotic and candidates for prebiotics on Clostridium difficile strains to adhere to various human epithelial cell lines and to compare the adhesive properties of specific C. difficile strains. We also sought to examine the effect of different concentrations of fructooligosaccharides and mannose on the formation of biofilms by C. difficile strains. The influence of cellobiose, fructooligosaccharides, inulin, mannose, and raffinose on the adherence properties of various C. difficile strains, including motile 630, non-motile M120, and 10 clinical motile ribotype 027 strains, to non-mucous secreting HT-29, mucous secreting HT-29 MXT, and CCD 841 CoN cells lines. The most effective prebiotics were used in biofilm formation assays. We demonstrated that all C. difficile strains adhered to all cell lines. However, the C. difficile M120 non-motile strain was statistically more likely to adhere to all three cell lines (CFU median, 40) compared to the motile strains (CFU median, 3; p < 0.001). Furthermore, among the carbohydrates examined, only fructooligosaccharides and mannose were found to significantly decrease adhesion (p < 0.001) of C. difficile strains. Alternatively, usin...Continue Reading

References

Nov 1, 1995·Critical Reviews in Food Science and Nutrition·J van LooG Smits
Jul 8, 1999·The Journal of Nutrition·K R Niness
Oct 3, 2003·FEMS Immunology and Medical Microbiology·Itzhak OfekNathan Sharon
Mar 28, 2009·Journal of Pediatric Gastroenterology and Nutrition·Mitchell B Cohen
Jun 17, 2009·Nature Reviews. Microbiology·Maja RupnikDale N Gerding
Sep 15, 2009·Journal of Bacteriology·Susan M TwineSusan M Logan
Dec 23, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mark MillerSharon Kelly
Aug 2, 2012·Infection and Immunity·Annie AubrySusan M Logan
Feb 6, 2014·PloS One·Ekaterina G SemenyukAdam Driks
Feb 24, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J FreemanUNKNOWN Pan-European Longitudinal Surveillance of Antibiotic Resistance among Prevalent Clostridium difficile Ribotypes' Study Group
Nov 5, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Hanna PituchUNKNOWN Polish Clostridium difficile Study Group
May 8, 2018·Anaerobe·Adam P Roberts, Wiep Klaas Smits

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Citations

Mar 7, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Dorota WultańskaHanna Pituch
Oct 22, 2020·Open Biology·Arturo Aguilar-RojasNancy Guillen
Jul 28, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Julio E Arce MirandaMa Gabriela Paraje

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Statistica

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