Fructose and glucose combined with free fatty acids induce metabolic disorders in HepG2 cell: a new model to study the impacts of high-fructose/sucrose and high-fat diets in vitro

Molecular Nutrition & Food Research
Liang ZhaoBaoping Ji

Abstract

This work investigated the underlying mechanism of high-fructose/sucrose and high-fat diets, which rapidly induce metabolic syndrome in vivo, via a new cell model. Glucose and/or fructose were used to induce the human hepatoma cell (HepG2) in the presence of palmitic acid (PA), oleic acid (OA), or combined fatty acids (CFA) for 24 h. The alterations in lipid and uric acid production, glucose metabolism, oxidative status, and related genes and proteins were monitored. The cell model that featured metabolic disorders was established by treatment of 10 mM glucose and 15 mM fructose plus 1 mM CFA. Results showed that PA mainly induced insulin resistance, oxidative stress, and triglyceride (TG) secretion, whereas OA mainly contributed to intracellular TG. Fructose was mainly responsible for uric acid and cholesterol production. In addition, fructose synergistically elevated the intra- and extracellular TG and extracellular malonaldehyde with glucose and CFA. Regulations of genes and proteins associated with carbohydrate metabolism and lipogenesis partially explained the action of fructose in inducing the metabolic disorders in cell. The combination of glucose, fructose, and CFA could successfully induce metabolic disorders in HepG2 ...Continue Reading

References

Nov 1, 1991·The American Journal of Clinical Nutrition·P RitzB Charbonnel
Oct 1, 1988·The American Journal of Clinical Nutrition·J C Cohen, R Schall
Aug 1, 1995·Biochemical Society Transactions·J D McGarry
Apr 6, 2002·American Journal of Physiology. Endocrinology and Metabolism·Yoshio NagaiAtsunori Kashiwagi
Jan 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·C D HOLDSWORTH, A M DAWSON
Aug 26, 2004·JAMA : the Journal of the American Medical Association·Matthias B SchulzeFrank B Hu
Dec 26, 2006·Chemico-biological Interactions·María José Gómez-LechónJosé-Enrique O'Connor
Jul 4, 2007·Nutrition Reviews·Angela C Rutledge, Khosrow Adeli
Jul 30, 2008·Archives of Internal Medicine·Julie R PalmerLynn Rosenberg
Oct 1, 2008·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H J ZhangG Z Zhang
Feb 12, 2009·Journal of Gastroenterology and Hepatology·Matteo RicchiPaola Loria
Oct 6, 2009·Biochemical and Biophysical Research Communications·Hyun-Young KooManabu T Nakamura
Jan 21, 2010·Physiological Reviews·Luc Tappy, Kim-Anne Lê
Apr 15, 2010·Annals of the New York Academy of Sciences·Kimber L Stanhope, Peter J Havel
May 18, 2010·Nutrition·Luc TappyNicolas Paquot
Jun 8, 2010·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·R D MattesD B Allison
Jan 8, 2011·The Journal of Endocrinology·Paul W CatonRoger Corder
Jan 22, 2011·Brain : a Journal of Neurology·Jun LuZi-feng Zhang
Jan 26, 2011·Lipids in Health and Disease·Danshan HuangAnthony P Heaney
Dec 2, 2011·The Journal of Nutritional Biochemistry·Kyoko Nomura, Toshikazu Yamanouchi
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takuji IshimotoRichard J Johnson
Nov 7, 2012·The Journal of Physiology·Veronique Douard, Ronaldo P Ferraris
Nov 16, 2012·PloS One·Miguel A LanaspaRichard J Johnson
Jul 3, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takuji IshimotoRichard J Johnson
Aug 22, 2014·Journal of the American College of Nutrition·Viranda H JayalathDavid J A Jenkins
Jun 18, 2015·Nature·Peter MirtschinkWilhelm Krek
Aug 28, 2015·Molecular Nutrition & Food Research·Sithandiwe E MazibukoChristo J F Muller

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Citations

Apr 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Priscila Silva FigueiredoPriscila Aiko Hiane
Oct 20, 2017·Cardiovascular Research·Anil BhattaRobert W Caldwell
Jan 3, 2019·International Journal of Food Sciences and Nutrition·Wenfeng LiQiang Zhao
Mar 19, 2019·The Journal of Physiology·Katherine E Pinnick, Leanne Hodson
Oct 29, 2018·European Journal of Nutrition·Ngoc Anh HoangHolger Steinbrenner
Feb 14, 2018·PloS One·Arti AhluwaliaElisabetta Iori
Aug 23, 2018·NPJ Systems Biology and Applications·Elaina M MaldonadoJ Bernadette Moore
Sep 13, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Yun Yeong LeeYun-Hoon Choung
Mar 31, 2021·Bioscience, Biotechnology, and Biochemistry·Chang WangXue-Gang Luo

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