Sep 1, 1983

Fructose bisphosphatase from Escherichia coli. Purification and characterization

Archives of Biochemistry and Biophysics
J Babul, V Guixé

Abstract

Escherichia coli fructose-1,6-bisphosphatase has been purified for the first time, using a clone containing an approximately 50-fold increased amount of the enzyme. The procedure includes chromatography in phosphocellulose followed by substrate elution and gel filtration. The enzyme has a subunit molecular weight of approximately 40,000 and in nondenaturing conditions is present in several aggregated forms in which the tetramer seems to predominate at low enzyme concentrations. Fructose bisphosphatase activity is specific for fructose 1,6-bisphosphate (Km of approximately 5 microM), shows inhibition by substrate above 0.05 mM, requires Mg2+ for catalysis, and has a maximum of activity around pH 7.5. The enzyme is susceptible to strong inhibition by AMP (50% inhibition around 15 microM). Phosphoenolpyruvate is a moderate inhibitor but was able to block the inhibition by AMP and may play an important role in the regulation of fructose bisphosphatase activity in vivo. Fructose 2,6-bisphosphate did not affect the rate of reaction.

  • References13
  • Citations31

References

  • References13
  • Citations31

Citations

Mentioned in this Paper

Alkalescens-Dispar Group
Fructose-1,6-Bisphosphatase
Substrate Specificity
5'-Adenylic Acid
Hydrogen-Ion Concentration
FBP1

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