Fructosyltransferase activity of a glucan-binding protein from Streptococcus mutans

Journal of General Microbiology
R R RussellC W Douglas

Abstract

Streptococcus mutans serotype c produces several extracellular proteins which bind to affinity columns of immobilized glucans. The proteins are three distinct glucosyltransferases and another glucan-binding protein (molecular weight 74000) which is now shown to be a fructosyltransferase. This enzyme is antigenically distinct and genetically independent of two other fructosyltransferases produced by the same organism. A mutant is described which lacks the glucan binding fructosyltransferase and has defective ability to form adherent colonies in the presence of sucrose. Although the production of glucans from sucrose results in the glucan binding protein becoming bound to the bacterial surface, and hence perhaps contributing to adherence, the fructans synthesized by the enzyme do not appear to contribute to this phenomenon.

Related Concepts

IslA protein, Leuconostoc citreum
Carrier Proteins
Polycose
Glucosyltransferases
Hexosyltransferase
Plasma Protein Binding Capacity
Serotyping
Streptococcus mutans

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