Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells

Biological Research
Constant Anatole PiemeAjit Kumar Saxena

Abstract

Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence microscopy. The rate of apoptosis and the cell cycle were measured using flow cytometry (FCM). The phytochemical analysis of the extract was carried with HPLC/MS methods. The phytochemical analysis of the extracts indicated the presence of four known polyphenols (Syringic acid, Juglon, Luteolin and Myricetin) in both fruits and barks of Z. heitzii but in different quantities. Syringic acid and Myricetin concentrations were between 17-21 fold higher in the fruits than the stem bark. Rhamnetin (393.3...Continue Reading

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Citations

May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ammad Ahmad FarooqiAnupam Bishayee
Jun 10, 2016·International Journal of Molecular Medicine·Yidong CaoDong Jia
Mar 3, 2017·Oxidative Medicine and Cellular Longevity·Navneet Kumar YadavR K Singh
Aug 31, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Jacques Joël EssogoConstant Anatole Pieme
Oct 19, 2017·International Journal of Oncology·Conglin YeBin Zhang
Nov 25, 2016·Biological Research·Nguyen Thi XuanNong Van Hai

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Modfit

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