Abstract
Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence microscopy. The rate of apoptosis and the cell cycle were measured using flow cytometry (FCM). The phytochemical analysis of the extract was carried with HPLC/MS methods. The phytochemical analysis of the extracts indicated the presence of four known polyphenols (Syringic acid, Juglon, Luteolin and Myricetin) in both fruits and barks of Z. heitzii but in different quantities. Syringic acid and Myricetin concentrations were between 17-21 fold higher in the fruits than the stem bark. Rhamnetin (393.3...Continue Reading
References
Jun 27, 2002·Advances in Experimental Medicine and Biology·Joe A VinsonNicole Sandone
Feb 26, 2004·Breast Cancer Research : BCR·Marilena KampaElias Castanas
May 7, 2004·Journal of Pharmaceutical and Biomedical Analysis·Xiaolan ZhuHongwei Hang
Apr 9, 2005·Journal of Ethnopharmacology·Guédé Noël ZirihiPhilippe Grellier
Jul 29, 2005·Critical Reviews in Food Science and Nutrition·Augustin ScalbertLiliana Jiménez
Dec 6, 2005·Chemical & Pharmaceutical Bulletin·Jean-Bernard BonguiElisabeth Seguin
Mar 17, 2006·Free Radical Biology & Medicine·Han-Ming Shen, Zheng-gang Liu
Mar 21, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ying-Tang HuangMin-Hsiung Pan
Mar 29, 2006·Cancer Cell International·Anissa BelkaidBorhane Annabi
Sep 2, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hsin-Ling YangYou-Cheng Hseu
Sep 16, 2006·Cellular & Molecular Biology Letters·Indrajit ChowdhuryGanapathy K Bhat
Jul 20, 2007·Apoptosis : an International Journal on Programmed Cell Death·Shashi BhushanJaswant Singh
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Benoît D'Autréaux, Michel B Toledano
Sep 18, 2007·Apoptosis : an International Journal on Programmed Cell Death·Fayaz MalikJaswant Singh
Dec 7, 2007·Biological & Pharmaceutical Bulletin·Kyungsu KangChu Won Nho
Jun 5, 2008·Journal of Agricultural and Food Chemistry·M Emília JuanJoana M Planas
Aug 12, 2008·Chemico-biological Interactions·Aneta RogalskaZofia Jóźwiak
Sep 23, 2008·Phytotherapy Research : PTR·Małgorzata Zielińska-Przyjemska, Ewa Ignatowicz
Mar 21, 2009·Journal of Applied Toxicology : JAT·Almudena GarcíaAna I Haza
Apr 18, 2009·Nutrition and Cancer·Yasmine S TouilGuy G Chabot
Nov 6, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S JinW H Zhao
Dec 30, 2009·Leukemia & Lymphoma·Chun-Yan PengXiao-Shan Liu
Dec 15, 2012·Molecular Pharmaceutics·Shashi BhushanA K Saxena
Jan 16, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Ting-Ting WangGui-Ju Sun
Jul 9, 2013·BMC Complementary and Alternative Medicine·Nanfack PaulineNgogang Yonkeu Jeanne
Citations
Jul 15, 2015·Oncology Reports·Xiaolong YuMin Dai
May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ammad Ahmad FarooqiAnupam Bishayee
Oct 9, 2018·Drug and Chemical Toxicology·Varinder KaurSatwinderjeet Kaur
Jun 10, 2016·International Journal of Molecular Medicine·Yidong CaoDong Jia
Jan 19, 2019·Oncology Letters·Lan LanHongmei Tang
Mar 3, 2017·Oxidative Medicine and Cellular Longevity·Navneet Kumar YadavR K Singh
Aug 31, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Jacques Joël EssogoConstant Anatole Pieme
Oct 19, 2017·International Journal of Oncology·Conglin YeBin Zhang
May 23, 2019·Molecular Medicine Reports·Tian GaoBin Zhang
Nov 25, 2016·Biological Research·Nguyen Thi XuanNong Van Hai