FSTL1 interacts with VIM and promotes colorectal cancer metastasis via activating the focal adhesion signalling pathway

Cell Death & Disease
Chuansha GuZuguo Li

Abstract

Follistatin-like protein 1 (FSTL1) has been reported to have both tumour-promoting and tumour-suppressive characters. However, the role of FSTL1 in colorectal cancer (CRC) remains unclear. Here we showed that FSTL1 expression was significantly up-regulated in CRC tissues compared with the paired normal tissues. In addition, the higher FSTL1 expression was associated with the infiltrating depth, lymph node metastasis and poor prognosis of CRC. Enhanced expression of FSTL1 distinctly increased cell migration and invasion in vitro, as well as promoting liver metastasis of CRC in vivo. Conversely, knockdown of FSTL1 expression significantly repressed invasion and metastasis of CRC. Mechanically, transcription factor Smad3 was involved in FSTL1 protein expression inducing by TGFβ1-Smad2/3 signalling. Furthermore, this effect of FSTL1 in promoting CRC progression was actualised via activating focal adhesions signalling pathway and regulating cytoskeleton rearrangement. We identified VIM, as an interactive protein of FSTL1, participated in FSTL1-mediated aggressive phenotype. We showed the role of FSTL1 in CRC and explored its transcription regulation and downstream signalling molecular mechanisms. In conclusion, our findings suggeste...Continue Reading

References

Mar 5, 2003·The Journal of Cell Biology·Datsun A HsiaDavid D Schlaepfer
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Satyajit K MitraDavid D Schlaepfer
Aug 3, 2005·Nature Reviews. Cancer·Gordon W McLeanMargaret C Frame
May 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sreekanth P ReddyM R Satyanarayana Rao
Jun 12, 2008·International Journal of Cancer. Journal International Du Cancer·Xiaojie TanGuangwen Cao
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suzanne D ClutterRaphael Hirsch
Apr 16, 2009·Journal of Cell Science·Ramona BhattacharyaJonathan C R Jones
Jan 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melissa G MendezRobert D Goldman
May 23, 2012·Cancer Discovery·Tsukasa ShibueRobert A Weinberg
Mar 22, 2013·Journal of Cell Science·Steven M FrischBenjamin Cieply
Aug 24, 2013·Cancer Research·Chie Kudo-SaitoYutaka Kawakami
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Mar 19, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
May 20, 2014·Immunologic Research·Yury ChalyRaphael Hirsch
Jun 4, 2014·Mediators of Inflammation·Joanna Magdalena Zarzynska
Aug 8, 2014·Nature Reviews. Cancer·Florian J SulzmaierDavid D Schlaepfer
Jan 15, 2015·The Journal of Experimental Medicine·Yingying DongWen Ning
Jul 29, 2015·Gastroenterology·John M Carethers, Barbara H Jung
Apr 5, 2016·Biochimica Et Biophysica Acta·Zhi-Yan HuZu-Guo Li
Jul 19, 2016·Experimental Gerontology·Luc Rochette, Catherine Vergely

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Citations

Apr 17, 2020·Cancer Biotherapy & Radiopharmaceuticals·Taobo JinTianya Zhang
Aug 23, 2018·Oncotarget·Gerolamo BevivinoGiovanni Monteleone
Oct 21, 2020·Infection and Immunity·Matthew HenkelBrian T Campfield
Feb 5, 2021·Cancers·Chie Kudo-SaitoYamato Ogiwara
Jul 25, 2021·Journal of Gastrointestinal Cancer·Kazumori AraiKo Ohata
Aug 28, 2021·Biomedicines·Olga K ParfenovaDmitry V Grishin

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Methods Mentioned

BETA
transfection
PCR
immunoprecipitation
Assay
ELISA
xenograft
ChIP
CoIP
transgenic

Software Mentioned

GSEA
Oncomine
CoIP
JARSPAR
Gene Set Enrichment Analysis ( GSEA )
SPSS

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