FT-IR spectroscopy for identification of closely related lactobacilli

Journal of Microbiological Methods
Astrid OustAchim Kohler

Abstract

Fourier Transform Infrared (FT-IR) spectroscopy was used to analyse 56 strains from four closely related species of Lactobacillus, L. sakei, L. plantarum, L. curvatus and L. paracasei. Hierarchical Cluster Analysis (HCA) was used to study the clusters in the data, but in the dendrogram, the spectra were not differentiated into four separate clusters corresponding to species. When the data were analysed with Partial Least Squares Regression (PLSR), the strains were differentiated into four clusters according to species. It was also possible to recognise strains that were incorrectly identified by conventional methods prior to the FT-IR analysis. PLSR was used to identify strains from three of the species, and the results were compared to two other multivariate methods, Soft Independent Modelling of Class Analogy (SIMCA) and K-Nearest Neighbour (KNN). The three methods gave equally good identification results. The results show that FT-IR spectroscopy in combination with PLSR, or other multivariate methods, is well suited for identification of Lactobacillus at the species level, even in quite large data sets.

References

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Citations

Jul 19, 2013·Applied Microbiology and Biotechnology·Mareike Wenning, Siegfried Scherer
Jan 5, 2006·Applied and Environmental Microbiology·Astrid OustAchim Kohler
Oct 6, 2010·Canadian Journal of Microbiology·Isabelle AdtGanesh D Sockalingum
Sep 19, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Shanmugaraj GowrishankarShunmugiah Karutha Pandian
Jul 16, 2014·Analytical and Bioanalytical Chemistry·Valber Elias de AlmeidaGermano Véras
Mar 8, 2011·Marine Environmental Research·Chari NithyaShunmugiah Karutha Pandian
Sep 14, 2006·Journal of Applied Microbiology·C SandtG D Sockalingum
Sep 4, 2015·Mycoses·Chandran SivasankarShunmugiah K Pandian
Jul 25, 2006·International Journal of Food Microbiology·Alejandra BoschOsvaldo Yantorno
Oct 2, 2014·Theriogenology·Isabella PrunnerMarc Drillich
Jun 22, 2006·Biochimica Et Biophysica Acta·Christoph KrafftReiner Salzer
Dec 7, 2007·FEMS Microbiology Letters·Astrid Oust JanbuAchim Kohler
Nov 10, 2017·Journal of Biophotonics·Valeria TafintsevaAchim Kohler
Nov 2, 2017·Biotechnology Journal·Cristina QuintelasClara Sousa
Jul 17, 2015·Foods·Mauricio I SantosAndrea Gomez-Zavaglia
Feb 11, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Satoshi FujiiShigeori Takenaka
Oct 23, 2019·Applied Microbiology and Biotechnology·Meng XuGuodong Zheng
May 7, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sajiya Yusuf MujawarSantosh Kumar Dubey
Jan 28, 2011·Journal of Agricultural and Food Chemistry·Jianhui XiaoXingguo Wang

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