PMID: 7547718Apr 1, 1995Paper

FTIR and NMR studies on the hydration of a high-M(r) subunit of glutenin

International Journal of Biological Macromolecules
Peter S BeltonA S Tatham

Abstract

The hydration behaviour of a purified high-M(r) subunit of glutenin has been studied using Fourier transform infra-red (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. The water-insoluble protein was examined in an unalkylated form with intermolecular disulfide bonds, and in a reduced and alkylated (unpolymerized) form. Hydration produced a marked increase in chain mobility, especially above a threshold water content of about 37% w/w. NMR experiments also showed that some parts of the chain were held in a much less mobile state, even at higher water contents. Little difference could be seen between alkylated and unalkylated subunits, implying that NMR is sensitive to localized motions, but not to any restrictions imposed by disulfide bridges close to the chain ends. FTIR spectra of the protein films have shown that increasing hydration enables changes to occur in favour of a more extended and beta-sheet-type structure. The changes in secondary structure are very noticeable at water contents corresponding to the NMR mobility threshold. The behaviour is influenced by intermolecular interactions. beta-sheet formation is enhanced by the presence of disulfide bonds in the unalkylated samples. There is little evidence of bet...Continue Reading

References

May 1, 1979·Biopolymers·G CareriE Gratton
Dec 1, 1990·Biopolymers·S D Kennedy, R G Bryant
Nov 1, 1967·The Journal of Physical Chemistry·C A Swenson, R Formanek
May 1, 1979·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P I PayneJ A Blackman

❮ Previous
Next ❯

Citations

Dec 4, 2003·Biochemical and Biophysical Research Communications·Sophie SouriceKhalil Elmorjani
Jul 18, 2001·Journal of the American Chemical Society·Y Z LeeW S Fann
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter R ShewryArthur S Tatham
Jan 10, 2012·Annual Review of Food Science and Technology·Jan A DelcourBert Lagrain
Nov 13, 2007·Journal of Food Science·Faqir Muhammad AnjumMuhammad Umair Arshad
May 4, 2011·Journal of Food Science·Anusooya S SivamConrad O Perera
Jul 28, 2004·Phytochemistry·David M GottliebIb Søndergaard
Nov 26, 2015·Food Chemistry·Toheder RahamanLata Ramchandran
Sep 26, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Cátia SalvadorA Teresa Caldeira
Sep 18, 1995·FEBS Letters·J HargreavesM A Hemminga
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Audrey L Girard, Joseph M Awika
Dec 31, 2020·International Journal of Molecular Sciences·Yi LiDong Yang
Jan 11, 2021·International Journal of Biological Macromolecules·Monika C WehrliMario Jekle
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Konrad KłosokAgnieszka Nawrocka
Sep 24, 2014·Journal of Agricultural and Food Chemistry·Xiaoming WangZhaoxin Lu
Sep 6, 2011·Biomacromolecules·Aman UllahJianping Wu
Jan 14, 2012·Journal of Agricultural and Food Chemistry·Carla D MejiaBruce R Hamaker
Aug 6, 2011·Journal of Agricultural and Food Chemistry·Mária OszvaldLászló Tamás
Jul 17, 2001·Journal of Agricultural and Food Chemistry·Y WangT R Noel
Feb 13, 2007·Biomacromolecules·Xiaoqing ZhangIko M Burgar
May 10, 2005·Biomacromolecules·Xiaoqing ZhangEleni Lourbakos
Apr 5, 2007·Biomacromolecules·Abdullah AlmutawahPeter S Belton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.