FTIR and XPS studies of protein adsorption onto functionalized bioactive glass

Biochimica Et Biophysica Acta
C GruianV Simon

Abstract

Adsorption and structural changes that occur upon interaction between methemoglobin (MetHb) and 5-methyl-aminomethyl-uridine forming enzyme (MnmE) with the surface of a bioactive glass (BG) were investigated by Fourier Transform Infrared (FTIR) spectroscopy and X-ray Photoelectron Spectroscopy (XPS). The effect of glutaraldehyde (GA) as a coupling agent for protein adsorption on BG was also investigated. The comparative analysis of FTIR spectra recorded from lyophilized proteins and from bioactive glass surface after protein adsorption was considered in order to obtain information about the changes in the secondary structure of the proteins. XPS data were used to determine the surface coverage. The unfolding of adsorbed proteins due to interactions between the internal hydrophobic protein domains and the hydrophobic BG surface was evidenced. After adsorption, the amount of α-helix decreases and less ordered structures (turns, random coils and aggregates) are preponderant. These changes are less pronounced on the BG functionalized with GA, suggesting that the treatment with GA preserves significantly larger amounts of α-helices in the structure of both proteins after adsorption.

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Citations

Jul 17, 2014·Colloids and Surfaces. B, Biointerfaces·Robert A Latour
Dec 27, 2015·Analytical Biochemistry·Jose O Esteves-Villanueva, Sanela Martic-Milne
Oct 10, 2015·Photochemistry and Photobiology·Ashwini A GhogareAlexander Greer
Apr 5, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Elizabeth J MartinKenneth R Shull
Feb 15, 2014·Colloids and Surfaces. B, Biointerfaces·Mahdi Dargahi, Sasha Omanovic
Aug 30, 2014·Journal of the Mechanical Behavior of Biomedical Materials·A EscudeiroA Cavaleiro
May 14, 2016·Bio-medical Materials and Engineering·K MagyariV Simon
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Martin KurnikKevin W Plaxco
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Aug 30, 2013·Journal of Biomaterials Applications·Emilia VaneaViorica Simon
Sep 15, 2017·Journal of Materials Science. Materials in Medicine·Tia J KowalMatthias M Falk
Aug 12, 2017·Journal of Applied Biomaterials & Functional Materials·Wai-Ching LiuTien-Min G Chu
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Sep 6, 2018··Kanokthip BoonyarattanakalinWeerachon Phoohinkong

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