FTIR microspectroscopic study of cell types and potential confounding variables in screening for cervical malignancies

Biospectroscopy
B R WoodD McNaughton

Abstract

FTIR microscopy was applied to the analysis of cell types and other variables present in Pap smears to ascertain the limitations of infrared spectroscopy in the diagnosis of cervical cancer and dysplasia. It was found that leukocytes, and in particular lymphocytes, have spectral features in the phosphodiester region (1300-900 cm[-1]) suggestive of what has previously been described as changes indicative of malignancy. Endocervical cells and fibroblasts have similar spectral features to HeLa cells and consequently could also confound diagnosis. The use of ethanol as a fixative and dehydrating agent results in retention of glycogen in cervical cell types and thus minimizes spectral changes in the glycogen region due to sampling technique. Spectra of seminal fluids exhibit strong bands in the phosphodiester/carbohydrate region; however, sperm contamination should be easily detectable by the presence of a distinctive doublet at 981/968 cm(-1). Erythrocyte spectra exhibit a reduction in glycogen band intensity, but can be discerned by a relatively low-intensity nu(s) PO2- band. Endocervical mucin spectra exhibit a reduction in glycogen bands and a very pronounced nu(s) PO2- band, which is similar in intensity to the corresponding ba...Continue Reading

References

Jan 1, 1991·Journal of General Microbiology·D HelmD Naumann
May 2, 1991·Nature·D NaumannH Labischinski
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B RigasP T Wong
Feb 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·C E MountfordP Russell
May 1, 1969·The Histochemical Journal·D Hopwood

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