FTIR spectral signature of the effect of cardiotonic steroids with antitumoral properties on a prostate cancer cell line

Biochimica Et Biophysica Acta
Régis GasperErik Goormaghtigh

Abstract

We show in the present work that the infrared (IR) spectrum of human PC-3 prostate cancer cells exposed to anticancer drugs could offer a unique opportunity to get a fingerprint of all the major biochemical components (DNA, RNA, proteins, lipids, etc.) present in the cells and to identify with high sensitivity the signature of the metabolic changes induced by anticancer drugs. We investigated here the FTIR-related signatures of the effect of 4 structurally-related cardiotonic steroids (CS), i.e. ouabain, 19-hydroxy-2″-oxovoruscharin, hellebrin and 19-hydroxy-hellebrin on PC-3 cancer cells incubated between 0 and 36 h in the absence (control) or the presence of the CS. For each molecule a single spectral signature described the largest part of the time dependent modifications with a possible very minor second component. The spectral signatures characterizing the effects of each of the four CS were unique but very similar when compared to the signature of the effect of an intercalating anticancer drug, i.e. doxorubicin, selected as a positive reference compound in our study, suggesting a fully distinct set of cellular perturbations. The current study thus illustrates that Fourier Transform Infrared (FTIR) analyses can be used to ...Continue Reading

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Citations

Oct 26, 2013·Biochimica Et Biophysica Acta·Allison DerenneErik Goormaghtigh
Sep 4, 2015·Biochimica Et Biophysica Acta·Alix MignoletErik Goormaghtigh
Apr 5, 2016·Food Chemistry·Haroon Elrasheid TahirAbdalbasit Adam Mariod
Jun 4, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Atul GuptaArvind S Negi
Oct 23, 2012·Biochimica Et Biophysica Acta·Allison DerenneErik Goormaghtigh
Dec 18, 2014·The Analyst·M VerdonckE Goormaghtigh
Jul 17, 2014·The Analyst·Graeme ClemensMatthew J Baker
Jun 8, 2012·The Analyst·Allison DerenneErik Goormaghtigh

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