PMID: 11313132Apr 21, 2001Paper

FTIR spectroscopic analysis of Saccharomyces cerevisiae cell walls: study of an anomalous strain exhibiting a pink-colored cell phenotype

FEMS Microbiology Letters
A GalichetMichel Manfait

Abstract

A new strain, exhibiting an intriguing pink-colored cell phenotype, was obtained after an encoding alpha-glucosidase gene from an archaebacteria Thermococcus hydrothermalis was cloned by functional complementation of a mal11 Saccharomyces cerevisiae mutant TCY70. The possible implications of the alpha-glucosidase on the cell wall were evaluated by infrared spectroscopy and data indicate a 30% decrease in mannoproteins and an increase in beta-glucans. The loss of mannoproteins was confirmed by experiments on cells deprived of peptidomannans. Modifications in the major components of the cell wall did not jeopardize cell viability. Such rapid optical spectroscopic method can be used to screen a wide range of yeast mutants.

References

Jan 1, 1991·Journal of General Microbiology·D HelmD Naumann
May 2, 1991·Nature·D NaumannH Labischinski
Jan 1, 1985·Current Topics in Medical Mycology·G H Fleet
Jul 1, 1994·Yeast·F M Klis
Mar 6, 1997·Biochemical and Biophysical Research Communications·G D SockalingumM Manfait
Jun 3, 1998·Applied and Environmental Microbiology·M KümmerleH Seiler
Jul 31, 1998·Journal of Bacteriology·P N Lipke, R Ovalle
Oct 9, 1998·Applied Microbiology and Biotechnology·T H NguyenP L Rogers
Jan 9, 1999·The Plant Journal : for Cell and Molecular Biology·L ChenM C McCann

❮ Previous
Next ❯

Citations

May 4, 2013·Journal of Agricultural and Food Chemistry·Phuong Dinh KimOlga Valentová
Jun 8, 2014·Carbohydrate Polymers·Eva MachováSlavomír Bystrický
Dec 3, 2014·Carbohydrate Polymers·Mariana PintoManuel A Coimbra
Dec 13, 2006·Biochimica Et Biophysica Acta·Robert J KarremanGeorge G Lindsey
Jun 5, 2016·Carbohydrate Polymers·Alžbeta ČížováSlavomír Bystrický
Feb 24, 2016·Mycopathologia·Suma C PemmarajuVikas Pruthi
Jan 25, 2017·Scientific Reports·Muhammad HaneefAthanassia Athanassiou
Sep 19, 2001·Yeast
Jan 24, 2014·Biotechnology for Biofuels·Diletta AmiPaola Branduardi
Sep 19, 2017·Applied Microbiology and Biotechnology·Zaman KhanMuhammad Arif
Feb 6, 2020·Scientific Reports·Starlin Péguy Engozogho AnrisBertrand Charrier
Nov 16, 2006·Analytical and Bioanalytical Chemistry·Adriana SzeghalmiKathleen M Gough
Sep 14, 2006·Journal of Applied Microbiology·C SandtG D Sockalingum
Dec 18, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Bzducha-WróbelEwa Majewska
Sep 9, 2017·Nanoscale·Fabienne QuilèsSofiane El-Kirat-Chatel
Mar 27, 2010·Journal of Applied Microbiology·N R BüchlS Scherer
Apr 8, 2015·Applied Microbiology and Biotechnology·Bipinchandra K SalunkeBeom Soo Kim
Jan 21, 2021·International Journal of Molecular Sciences·Ionut Avramia, Sonia Amariei
Jan 26, 2021·3 Biotech·Gildomar Lima Valasques JuniorSandra Aparecida de Assis
Jan 12, 2021·Saudi Journal of Biological Sciences·Rajapandiyan KrishnamoorthyAli A Alshatwi
May 19, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Wenqian LiYan Yu
Apr 1, 2021·Applied Biochemistry and Biotechnology·Natthaporn ChotigavinSanya Kudan
Jan 7, 2010·Journal of Agricultural and Food Chemistry·Matteo CavagnaSandra Torriani

❮ Previous
Next ❯

Related Concepts