FTIR Spectroscopic Study of the Secondary Structure of Globular Proteins in Aqueous Protic Ionic Liquids

Frontiers in Chemistry
Radhika ArunkumarTamar L Greaves

Abstract

Protein misfolding is a detrimental effect which can lead to the inactivation of enzymes, aggregation, and the formation of insoluble protein fibrils called Amyloids. Consequently, it is important to understand the mechanism of protein folding, and under which conditions it can be avoided or mitigated. Ionic liquids (ILs) have previously been shown as capable of increasing or decreasing protein stability, depending on the specific IL, IL concentration and which protein. However, a greater range of IL-proteins need to be systematically explored to enable the development of structure-property relationships. In this work, the secondary structure of four proteins, lysozyme, trypsin, β-lactoglobulin and α-amylase, were studied in aqueous solutions of 10 protic ionic liquids (PILs) with 0-50 mol% PIL present. The PILs consisted of ethyl-, ethanol-, diethanol- and triethanolammonium cations paired with nitrate, formate, acetate or glycolate anions. The secondary structure was obtained using ATR-FTIR spectroscopy. It was found that lysozyme and trypsin retained its secondary structure, consistent with a native folded state, for many of the aqueous IL solutions which contained a formate or nitrate anion at the most dilute concentrations...Continue Reading

References

Nov 1, 1983·Archives of Biochemistry and Biophysics·L K CreamerG N Malcolm
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S N Timasheff
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R V Rariy, A M Klibanov
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KnubovetsA M Klibanov
Feb 10, 2000·Acta Crystallographica. Section D, Biological Crystallography·J A GarlitzG E Borgstahl
Jul 19, 2002·Biophysical Journal·Alessandro PaciaroniGiuseppe Onori
Oct 10, 2002·Journal of the American Chemical Society·Barbara HribarKen A Dill
Aug 9, 2003·Physical Review Letters·Ariel Fernández, L Ridgway Scott
May 1, 1962·Archives of Biochemistry and Biophysics·F F BUCKF F NORD
Jan 14, 2004·Protein Science : a Publication of the Protein Society·Aoneng CaoLuhua Lai
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Nick PaceJ Martin Scholtz
Sep 30, 2005·Chemical Communications : Chem Comm·Kyoko FujitaMaria Forsyth
Nov 9, 2007·The Journal of Physical Chemistry. B·R MancinelliA K Soper
Dec 22, 2007·Chemical Reviews·Tamar L Greaves, Calum J Drummond
Feb 20, 2009·Chemical Communications : Chem Comm·Nolene Byrne, C Austen Angell
Jul 3, 2009·Journal of the Royal Society, Interface·Maria Grazia OrtoreDaniela Russo
Dec 23, 2009·Biochimica Et Biophysica Acta·Zhen YangPeter J Halling
Dec 15, 2010·Physical Chemistry Chemical Physics : PCCP·Pankaj AttriAnil Kumar
Jan 12, 2011·Annual Review of Physical Chemistry·Jeremy L England, Gilad Haran
Nov 18, 2011·Physical Chemistry Chemical Physics : PCCP·Hermann WeingärtnerChristian Herrmann
Jan 14, 2012·The Journal of Chemical Physics·Christoph GögeleinStefan U Egelhaaf
Aug 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Kathrin EngelhardtBjörn Braunschweig
Jul 6, 2014·International Journal of Pharmaceutics·Priscilla KheddoAlexander P Golovanov
Mar 8, 2016·Journal of Chemical Technology and Biotechnology·Hua Zhao
Oct 11, 2016·Physical Chemistry Chemical Physics : PCCP·Emmy C WijayaTamar L Greaves
Feb 9, 2017·Topics in Current Chemistry·Christian Schröder

❮ Previous
Next ❯

Citations

Jan 31, 2020·Critical Reviews in Food Science and Nutrition·Janine WagnerThomas Moschakis
Jan 14, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuri D IvanovVadim S Ziborov
Feb 18, 2021·Journal of Colloid and Interface Science·Qi HanTamar L Greaves
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hasan Tanvir ImamAndrew Craig Marr
Jan 21, 2022·ACS Biomaterials Science & Engineering·Douglas S MacPhersonRein V Ulijn

❮ Previous
Next ❯

Methods Mentioned

BETA
Infrared Spectroscopy
circular dichroism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.