Fto Deficiency Reduces Anxiety- and Depression-Like Behaviors in Mice via Alterations in Gut Microbiota

Theranostics
Lijuan SunYongzhan Nie

Abstract

Depression and obesity have high concurrence within individuals, which may be explained by sharing the same risk factors, including disruption of the intestinal microbiota. However, evidence that delineated the causal connections is extremely scarce. Methods: Mice lacking fat mass- and obesity-associated gene (Fto) were generated. Fto-deficient and wild-type control mice were subjected to novel conditions with or without chronic unpredictable mild stress (CUMS) for 6 weeks. Some mice were treated with antibiotics via their drinking water for 6 weeks in order to deplete their microbiota. Behavioral tests were performed to evaluate anxiety- and depression-like behaviors. 16S rRNA amplicon and metagenomic sequencing were employed to analyse fecal microbiota. Plasma levels of inflammatory cytokines and lipopolysaccharides (LPS) were also compared. Results: Deletion of Fto led to lower body weight and decreased anxiety- and depression-like behaviors, Fto+/- mice were also less susceptible to stress stimulation, highlighting the essential role of Fto in pathogenesis of depression. With regard to gut microbiota, Fto deficiency mice harbored specific bacterial signature of suppressing inflammation, characterized with higher abundance o...Continue Reading

Citations

Sep 3, 2019·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Jiamin WuXiang Zhong
Feb 23, 2020·Epigenomics·Andrew M ShafikPeng Jin
Aug 26, 2020·Molecular Biotechnology·R Karthiya, Piyush Khandelia
Jul 19, 2020·Current Medical Science·Bing Han, Hong-Hong Yao
Sep 25, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anil K ChokkallaRaghu Vemuganti
Sep 6, 2019·Helicobacter·Armelle Ménard, Annemieke Smet
Aug 24, 2019·Neuroscience·P K AnnapoornaArvind Kumar
Feb 1, 2021·Brain, Behavior, and Immunity·A M HamiehF Marchand
Dec 18, 2020·Frontiers in Aging Neuroscience·Ji Yeon ChungJuhyun Song
Apr 4, 2021·Microorganisms·Harish NarasimhanKristyn E Sylvia
May 22, 2021·Neuroscience and Biobehavioral Reviews·Juan Antonio Zarza-RebolloMargarita Rivera
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Godwin SokporTran Tuoc
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Jiahui LuoKai Sun

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Methods Mentioned

BETA
Protein Assay
ELISA
Assay
PCR
16S sequencing
amplicon sequencing
environmental stress

Software Mentioned

SPSS
QIIME

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