FtsA acts through FtsW to promote cell wall synthesis during cell division in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America
Kyung-Tae ParkJoe Lutkenhaus

Abstract

In Escherichia coli, FtsQLB is required to recruit the essential septal peptidoglycan (sPG) synthase FtsWI to FtsA, which tethers FtsZ filaments to the membrane. The arrival of FtsN switches FtsQLB in the periplasm and FtsA in the cytoplasm from a recruitment role to active forms that synergize to activate FtsWI. Genetic evidence indicates that the active form of FtsQLB has an altered conformation with an exposed domain of FtsL that acts on FtsI to activate FtsW. However, how FtsA contributes to the activation of FtsW is not clear, as it could promote the conformational change in FtsQLB or act directly on FtsW. Here, we show that the overexpression of an activated FtsA (FtsA*) bypasses FtsQ, indicating it can compensate for FtsQ's recruitment function. Consistent with this, FtsA* also rescued FtsL and FtsB mutants deficient in FtsW recruitment. FtsA* also rescued an FtsL mutant unable to deliver the periplasmic signal from FtsN, consistent with FtsA* acting on FtsW. In support of this, an FtsW mutant was isolated that was rescued by an activated FtsQLB but not by FtsA*, indicating it was specifically defective in activation by FtsA. Our results suggest that in response to FtsN, the active form of FtsA acts on FtsW in the cytopl...Continue Reading

References

Jul 1, 1997·Molecular Microbiology·S G AddinallJ Lutkenhaus
Feb 16, 2002·The EMBO Journal·Sebastien Pichoff, Joe Lutkenhaus
Nov 9, 2002·FEMS Microbiology Letters·Beatriz Lara, Juan A Ayala
Mar 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brett GeisslerWilliam Margolin
Jul 12, 2005·Current Biology : CB·Nathan W Goehring, Jon Beckwith
May 16, 2007·Molecular Microbiology·Sebastien Pichoff, Joe Lutkenhaus
Oct 17, 2007·The Journal of Biological Chemistry·Patrick MüllerWaldemar Vollmer
Apr 29, 2008·Journal of Bacteriology·Audrey Le GouëllecAndré Zapun
Oct 27, 2009·Journal of Bacteriology·Joe Lutkenhaus
Feb 20, 2010·The EMBO Journal·Fusinita van den EntJan Löwe
Nov 26, 2010·Molecular Microbiology·Nelly DubarryFrançois-Xavier Barre
Oct 8, 2013·Current Opinion in Microbiology·Piotr Szwedziak, Jan Löwe
Aug 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shishen DuJoe Lutkenhaus
Aug 16, 2016·Nature·Alexander J MeeskeDavid Z Rudner
Jul 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sebastien PichoffJoe Lutkenhaus
Jan 30, 2019·Journal of Bacteriology·Bing LiuPiet A J de Boer
Jun 7, 2019·Trends in Microbiology·Shishen Du, Joe Lutkenhaus

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.