PMID: 9658012Jul 11, 1998Paper

ftsE(Ts) affects translocation of K+-pump proteins into the cytoplasmic membrane of Escherichia coli

Journal of Bacteriology
H UkaiA Nishimura

Abstract

The ftsE(Ts) mutation of Escherichia coli causes defects in cell division and cell growth. We expressed alkaline phosphatase (PhoA) fusion proteins of KdpA, Kup, and TrkH, all of which proved functional in vivo as K+ ion pumps, in the mutant cells. During growth at 41 degrees C, these proteins were progressively lost from the membrane fraction. The reduction in the abundance of these proteins inversely correlated with cell growth, but the preformed proteins in the membrane were stable at 41 degrees C, indicating that the molecules synthesized at the permissive temperature were diluted in a growth-dependent manner at a high temperature. Pulse-chase experiments showed that KdpA-PhoA was synthesized, but the synthesized protein did not translocate into the membrane of the ftsE(Ts) cells at 41 degrees C and degraded very rapidly. The loss of KdpA-PhoA from the membrane fractions of ftsE(Ts) cells was suppressed by a multicopy plasmid carrying the ftsE+ gene. While cell growth stopped when the abundance of these proteins decreased 15-fold, the addition of a high concentration of K+ ions specifically alleviated the growth defect of ftsE(Ts) cells but not cell division, and the cells elongated more than 100-fold. We conclude that one ...Continue Reading

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Citations

Feb 22, 2011·Current Microbiology·Sougata RoyParthasarathi Ajitkumar
Dec 17, 2014·The Protein Journal·Mushtaq Ahmad MirParthasarathi Ajitkumar
Jan 20, 2004·Journal of Bacteriology·Kari L SchmidtDavid S Weiss
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Mark J McBride
Feb 27, 1999·Molecular Microbiology·E de LeeuwJ Luirink
Apr 21, 2009·Journal of Bacteriology·S J Ryan ArendsDavid S Weiss
Sep 7, 2001·Annual Review of Microbiology·M J McBride
Jan 18, 2006·Archives of Microbiology·Mushtaq Ahmad MirParthasarathi Ajitkumar
Aug 29, 2013·FEMS Microbiology Letters·Viswanatha KrishnakumariRamakrishnan Nagaraj

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