FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins.

The Journal of Biological Chemistry
Yoshinori AkiyamaK Ito

Abstract

The FtsH protein is a membrane-bound ATPase of Escherichia coli that was proposed to be involved in membrane protein assembly as well as degradation of some unstable proteins. SecY, a subunit of protein translocase, is FtsH dependently degraded in vivo when it fails to associate with its partner (the SecE protein). We constructed a series of mutants in which mutations were introduced into conserved residues in the two ATP binding consensus sequences or the zinc binding sequence of FtsH. We purified wild-type and mutant FtsH proteins by making use of a polyhistidine tag attached to their carboxyl termini. Complementation analysis and ATPase activity assays in vitro indicated that, of the two sets of ATP binding sequence motifs, the one located C-terminally (A1) is essential for ATPase activity and in vivo functioning of FtsH. Wild-type FtsH protein degraded purified SecY in an ATP hydrolysis-dependent manner in vitro. Mutant proteins without ATPase activity were inactive in proteolysis. A zinc binding motif mutant showed a decreased proteolytic activity. SecY and FtsH were cross-linkable with each other in the membrane, provided that FtsH had an ATPase-inactivating mutation. These results demonstrate that FtsH binds to and degra...Continue Reading

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J AkimaruS Mizushima
Mar 16, 1990·Biochemical and Biophysical Research Communications·Y Akiyama, K Ito
Sep 1, 1986·Analytical Biochemistry·K M ChanK D Junger
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Oct 6, 1995·The Journal of Biological Chemistry·Y AkiyamaK Ito
Aug 25, 1995·The Journal of Biological Chemistry·K DouvilleW Wickner
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·C HermanP Bouloc
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A KiharaK Ito
Mar 10, 1995·The Journal of Biological Chemistry·T ShimoikeK Ito
Oct 31, 1994·FEBS Letters·N M Hooper
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·C K Murphy, J Beckwith
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·T BabaK Ito
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C HermanP Bouloc
Mar 1, 1993·Journal of Bacteriology·T TomoyasuT Ogura

❮ Previous
Next ❯

Citations

Aug 29, 2001·FEMS Microbiology Reviews·K H van WelyA J Driessen
Feb 3, 1998·Trends in Microbiology·I J van der Klei, M Veenhuis
Oct 2, 2012·Nature Reviews. Drug Discovery·Ravikiran M RajuEric J Rubin
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Wataru SakamotoUNKNOWN Sodmergen
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samuel WagnerJorge E Galán
Jun 5, 1999·The EMBO Journal·A KiharaK Ito
Sep 9, 2008·Journal of Bacteriology·Chen Katz, Eliora Z Ron
Feb 26, 2008·Journal of Bacteriology·Louise BaarsJan-Willem de Gier
Aug 10, 2002·Journal of Bacteriology·Shinobu ChibaKoreaki Ito
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Apr 9, 2008·Microbial Cell Factories·Jessica C ZweersJan Maarten van Dijl
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·A KiharaK Ito
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Danielle N MargalitDebabrata RayChaudhuri
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoshinori Akiyama
Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kyoko SuefujiDebabrata RayChaudhuri
Feb 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christoph BieniossekUlrich Baumann
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W de GierG von Heijne
Jun 5, 2012·Applied Microbiology and Biotechnology·Laurence Van OudenhoveBart Devreese
Mar 19, 2013·The Journal of Biological Chemistry·Wen LiParjit Kaur
Aug 10, 2000·The Journal of Cell Biology·H G Koch, M Müller
Jan 13, 2015·Journal of Molecular Biology·Franka ScharfenbergVikram Alva
May 12, 2010·Journal of Structural Biology·Sara Ayuso-TejedorJavier Sancho
Apr 27, 2010·Journal of Molecular Biology·Allison FayJonathan Dworkin
Sep 12, 2009·Research in Microbiology·Franz NarberhausSina Langklotz
Jul 22, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yuka Matsushita-IshiodoriTeru Ogura
Jul 3, 2008·Protein Science : a Publication of the Protein Society·A James LinkGeorge Georgiou
Jul 31, 2002·Biochemical and Biophysical Research Communications·Hisayoshi MakyioMasasuke Yoshida
Apr 9, 2011·Journal of Medical Microbiology·Shanmugam HemaiswaryaMukesh Doble
Feb 13, 2003·Molecular Microbiology·Linda FröderbergJan-Willem L De Gier

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.